Roch F, Serras F, Cifuentes F J, Corominas M, Alsina B, Amorós M, López-Varea A, Hernández R, Guerra D, Cavicchi S, Baguñá J, García-Bellido A
Centro de Biología Molecular Severo Ochoa, Madrid, Spain.
Mol Gen Genet. 1998 Jan;257(2):103-12. doi: 10.1007/pl00008620.
We have carried out screens for lethal mutations on the second chromosome of Drosophila melanogaster that are associated with abnormal imaginal disc morphologies, particularly in the wing disc. From a collection of 164 P element-induced mutations with a late larva/pupa lethal phase we have identified 56 new loci whose gene products are required for normal wing disc development and for normal morphology of other larval organs. Genetic mosaics of these 56 mutant lines show clonal mutant phenotypes for 23 cell-viable mutations. These phenotypes result from altered cell parameters. Causal relationships between disc and clonal phenotypes are discussed.
我们对黑腹果蝇第二条染色体上与异常成虫盘形态相关的致死突变进行了筛选，特别是在翅盘中。从164个具有晚期幼虫/蛹致死期的P因子诱导突变体库中，我们鉴定出56个新位点，其基因产物是正常翅盘发育和其他幼虫器官正常形态所必需的。这56个突变系的遗传嵌合体显示出23个细胞存活突变的克隆突变表型。这些表型是由细胞参数改变引起的。文中讨论了盘状表型和克隆表型之间的因果关系。
