果蝇黑腹果蝇第二条染色体上的P-lacW插入诱变：具有不同过度生长表型的致死突变体的分离
P-lacW insertional mutagenesis on the second chromosome of Drosophila melanogaster: isolation of lethals with different overgrowth phenotypes.
作者信息
Török T, Tick G, Alvarado M, Kiss I
机构信息
Institute of Genetics, Hungarian Academy of Sciences, Szeged.
出版信息
Genetics. 1993 Sep;135(1):71-80. doi: 10.1093/genetics/135.1.71.
Abstract
A single P-element insertional mutagenesis experiment was carried out for the second chromosome of Drosophila melanogaster using the P-lacW transposon. Out of 15,475 insertions on the second chromosome, 2,308 lethal and 403 semilethal mutants (altogether 2,711) were recovered. After eliminating clusters, 72% of the mutants represent independent insertions. Some of the mutants with larval, prepupal or pupal lethal phases have a prolonged larval period and show gradual overgrowth of the imaginal discs, brain and/or the hematopoietic organs (lymph glands). In this paper, 16 overgrowth mutants are described. As revealed by in situ hybridization, none of the mutations corresponds to any of the previously known overgrowth mutations on the second chromosome.
摘要
利用P-lacW转座子对黑腹果蝇的第二条染色体进行了单P因子插入诱变实验。在第二条染色体上的15475个插入中，回收了2308个致死突变体和403个半致死突变体（共2711个）。在消除聚类后，72%的突变体代表独立插入。一些具有幼虫、蛹前期或蛹期致死阶段的突变体幼虫期延长，并显示出成虫盘、脑和/或造血器官（淋巴腺）逐渐过度生长。本文描述了16个过度生长突变体。原位杂交显示，这些突变均与第二条染色体上任何先前已知的过度生长突变不对应。
相似文献
1
P-lacW insertional mutagenesis on the second chromosome of Drosophila melanogaster: isolation of lethals with different overgrowth phenotypes.果蝇黑腹果蝇第二条染色体上的P-lacW插入诱变：具有不同过度生长表型的致死突变体的分离
Genetics. 1993 Sep;135(1):71-80. doi: 10.1093/genetics/135.1.71.
2
Screening of larval/pupal P-element induced lethals on the second chromosome in Drosophila melanogaster: clonal analysis and morphology of imaginal discs.黑腹果蝇第二条染色体上幼虫/蛹期P因子诱导致死突变体的筛选：成虫盘的克隆分析及形态学研究
Mol Gen Genet. 1998 Jan;257(2):103-12. doi: 10.1007/pl00008620.
3
Phenotypes Associated with Second Chromosome P Element Insertions in Drosophila melanogaster.与黑腹果蝇第二条染色体P因子插入相关的表型
G3 (Bethesda). 2016 Aug 9;6(8):2665-70. doi: 10.1534/g3.116.030940.
4
Lethal(1) aberrant immune response mutations leading to melanotic tumor formation in Drosophila melanogaster.导致黑腹果蝇形成黑色素瘤的致死（1）异常免疫反应突变。
Dev Genet. 1991;12(3):173-87. doi: 10.1002/dvg.1020120302.
5
Evidence of mobilization of hobo transposons in a P-element mutagenesis screen.在P因子诱变筛选中hobo转座子激活的证据。
Genome. 1993 Dec;36(6):1138-47. doi: 10.1139/g93-152.
6
The congested-like tracheae gene of Drosophila melanogaster encodes a member of the mitochondrial carrier family required for gas-filling of the tracheal system and expansion of the wings after eclosion.果蝇的类充血气管基因编码线粒体载体家族的一个成员，该成员对于气管系统的气体填充以及羽化后翅膀的展开是必需的。
Genetics. 1997 Dec;147(4):1755-68. doi: 10.1093/genetics/147.4.1755.
7
patufet, the gene encoding the Drosophila melanogaster homologue of selenophosphate synthetase, is involved in imaginal disc morphogenesis.帕图费特基因编码果蝇硒磷酸合成酶的同源物，它参与成虫盘形态发生。
Mol Gen Genet. 1998 Jan;257(2):113-23. doi: 10.1007/s004380050630.
8
Genetic and molecular analysis in the 70CD region of the third chromosome of Drosophila melanogaster.黑腹果蝇第三条染色体70CD区域的遗传与分子分析。
Gene. 2000 Apr 4;246(1-2):157-67. doi: 10.1016/s0378-1119(00)00066-4.
9
Mutations in the Drosophila gene encoding ribosomal protein S6 cause tissue overgrowth.果蝇中编码核糖体蛋白S6的基因发生突变会导致组织过度生长。
Mol Cell Biol. 1993 Apr;13(4):2524-35. doi: 10.1128/mcb.13.4.2524-2535.1993.
10
P-element insertion alleles of essential genes on the third chromosome of Drosophila melanogaster: correlation of physical and cytogenetic maps in chromosomal region 86E-87F.黑腹果蝇第三条染色体上必需基因的P因子插入等位基因：染色体区域86E - 87F中物理图谱与细胞遗传学图谱的相关性
Genetics. 1997 Dec;147(4):1697-722. doi: 10.1093/genetics/147.4.1697.
引用本文的文献
1
Genetic Mapping of , a New Lethal Allele of .（某个基因）新的致死等位基因（的）遗传图谱绘制 。
你提供的原文不太完整，括号部分是推测补充以使句子完整，若有错误请指出以便我更准确翻译。
MicroPubl Biol. 2024 Jul 29;2024. doi: 10.17912/micropub.biology.001236. eCollection 2024.
2
One hundred years of cancer research: no longer in solitude.癌症研究百年：不再孤军奋战。
Dis Model Mech. 2019 Apr 1;12(4):dmm039032. doi: 10.1242/dmm.039032.
3
Diversification of heart progenitor cells by EGF signaling and differential modulation of ETS protein activity.通过 EGF 信号和 ETS 蛋白活性的差异调节实现心脏祖细胞的多样化。
Elife. 2018 Jun 5;7:e32847. doi: 10.7554/eLife.32847.
4
Search for Drosophila genes based on patterned expression of mini-white reporter gene of a P lacW vector in adult eyes.基于P lacW载体的微型白色报告基因在成年果蝇眼睛中的模式表达来搜索果蝇基因。
Rouxs Arch Dev Biol. 1995 Nov;205(3-4):114-121. doi: 10.1007/BF00357757.
5
lolal Is an Evolutionarily New Epigenetic Regulator of dpp Transcription during Dorsal-Ventral Axis Formation.Lolal是背腹轴形成过程中dpp转录的一种进化上新的表观遗传调节因子。
Mol Biol Evol. 2016 Oct;33(10):2621-32. doi: 10.1093/molbev/msw132. Epub 2016 Jul 8.
6
Cropped, Drosophila transcription factor AP-4, controls tracheal terminal branching and cell growth.截短型果蝇转录因子AP-4控制气管末端分支和细胞生长。
BMC Dev Biol. 2015 Apr 15;15:20. doi: 10.1186/s12861-015-0069-6.
7
Disruption of the protein kinase N gene of drosophila melanogaster results in the recessive delorean allele (pkndln) with a negative impact on wing morphogenesis.果蝇蛋白激酶 N 基因的破坏导致隐性 de 洛伦（pkndln）等位基因的产生，对翅膀形态发生有负面影响。
G3 (Bethesda). 2014 Apr 16;4(4):643-56. doi: 10.1534/g3.114.010579.
8
Drosophila Spag is the homolog of RNA polymerase II-associated protein 3 (RPAP3) and recruits the heat shock proteins 70 and 90 (Hsp70 and Hsp90) during the assembly of cellular machineries.果蝇 Spag 是 RNA 聚合酶 II 相关蛋白 3（RPAP3）的同源物，在细胞机器组装过程中招募热休克蛋白 70 和 90（Hsp70 和 Hsp90）。
J Biol Chem. 2014 Feb 28;289(9):6236-47. doi: 10.1074/jbc.M113.499608. Epub 2014 Jan 6.
9
A mosaic genetic screen for genes involved in the early steps of Drosophila oogenesis.用于鉴定果蝇卵子发生早期步骤相关基因的镶嵌遗传筛选。
G3 (Bethesda). 2013 Mar;3(3):409-25. doi: 10.1534/g3.112.004747. Epub 2013 Mar 1.
10
Division of labor: subsets of dorsal-appendage-forming cells control the shape of the entire tube.分工：背附肢形成细胞的子集控制整个管的形状。
Dev Biol. 2010 Oct 1;346(1):68-79. doi: 10.1016/j.ydbio.2010.07.018. Epub 2010 Jul 24.
本文引用的文献
1
The initiation of pupariation in Drosophila: dependence on growth of the imaginal discs.果蝇化蛹的起始：依赖于成虫盘的生长。
J Embryol Exp Morphol. 1980 Jun;57:155-65.
2
Intrinsic and extrinsic control of growth in developing organs.发育中器官生长的内在和外在控制。
Q Rev Biol. 1984 Dec;59(4):387-415. doi: 10.1086/414040.
3
Analysis of chromosome 4 in Drosophila melanogaster. II. Ethyl methanesulfonate induced lethals.黑腹果蝇4号染色体的分析。II. 甲磺酸乙酯诱导的致死突变
Genetics. 1971 Feb;67(2):235-52. doi: 10.1093/genetics/67.2.235.
4
Imaginal disc abnormalities in lethal mutants of Drosophila.果蝇致死突变体中的成虫盘异常。
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2594-8. doi: 10.1073/pnas.68.10.2594.
5
The anatomy and function of a segment of the X chromosome of Drosophila melanogaster.黑腹果蝇X染色体一段的解剖结构与功能。
Genetics. 1972 May;71(1):139-56. doi: 10.1093/genetics/71.1.139.
6
Physiological genetics of melanotic tumours in Drosophila melanogaster. VII. The relationship of dietary sterols to tumour penetrance.黑腹果蝇黑色素瘤的生理遗传学。VII. 膳食固醇与肿瘤发生率的关系。
Genet Res. 1972 Dec;20(3):317-29. doi: 10.1017/s0016672300013835.
7
Molecular cloning of lethal(2)giant larvae, a recessive oncogene of Drosophila melanogaster.致死（2）巨幼虫基因的分子克隆，果蝇的一个隐性癌基因。
EMBO J. 1985 Jun;4(6):1551-7. doi: 10.1002/j.1460-2075.1985.tb03816.x.
8
Mutations at the fat locus interfere with cell proliferation control and epithelial morphogenesis in Drosophila.果蝇中脂肪基因座的突变会干扰细胞增殖控制和上皮形态发生。
Dev Biol. 1988 Oct;129(2):541-54. doi: 10.1016/0012-1606(88)90399-5.
9
Structure of the l(2)gl gene of Drosophila and delimitation of its tumor suppressor domain.果蝇l(2)gl基因的结构及其肿瘤抑制结构域的界定。
Cell. 1987 Jul 17;50(2):215-25. doi: 10.1016/0092-8674(87)90217-0.
10
P-element-induced control mutations at the r gene of Drosophila melanogaster.P 因子诱导的黑腹果蝇 r 基因控制突变
Mol Cell Biol. 1985 Oct;5(10):2567-74. doi: 10.1128/mcb.5.10.2567-2574.1985.
Zotero 插件