Török T, Tick G, Alvarado M, Kiss I
Institute of Genetics, Hungarian Academy of Sciences, Szeged.
Genetics. 1993 Sep;135(1):71-80. doi: 10.1093/genetics/135.1.71.
A single P-element insertional mutagenesis experiment was carried out for the second chromosome of Drosophila melanogaster using the P-lacW transposon. Out of 15,475 insertions on the second chromosome, 2,308 lethal and 403 semilethal mutants (altogether 2,711) were recovered. After eliminating clusters, 72% of the mutants represent independent insertions. Some of the mutants with larval, prepupal or pupal lethal phases have a prolonged larval period and show gradual overgrowth of the imaginal discs, brain and/or the hematopoietic organs (lymph glands). In this paper, 16 overgrowth mutants are described. As revealed by in situ hybridization, none of the mutations corresponds to any of the previously known overgrowth mutations on the second chromosome.
利用P-lacW转座子对黑腹果蝇的第二条染色体进行了单P因子插入诱变实验。在第二条染色体上的15475个插入中，回收了2308个致死突变体和403个半致死突变体（共2711个）。在消除聚类后，72%的突变体代表独立插入。一些具有幼虫、蛹前期或蛹期致死阶段的突变体幼虫期延长，并显示出成虫盘、脑和/或造血器官（淋巴腺）逐渐过度生长。本文描述了16个过度生长突变体。原位杂交显示，这些突变均与第二条染色体上任何先前已知的过度生长突变不对应。
