Van Vleet J F, Ruth G, Ferrans V J
Am J Vet Res. 1976 Aug;37(8):911-22.
Light and electron microscopic studies were made of lesions which developed in skeletal muscles of 24 of 38 young growing pigs fed a semisynthetic diet deficient in selenium and vitamin E for 13 to 59 days. The major alterations in injured fibers progressed from hyaline degeneration, with subsequent macrophagic invasion and phagocytosis of disrupted sarcoplasm, to muscle fiber regeneration by myoblastic proliferation, fusion, and differentiation into fibers with mature myofibrils. The earliest electron microscopic alterations were myofibrillar lysis and disruption, with thick filaments persisting longest. Disruption of mitochondria, sarcoplasmic reticulum, and plasma membranes occurred in fibers with myofibrillar alterations. The basal lamina of the sarcolemma remained after destruction of the enclosed sarcoplasm and served as a scaffold for subsequent regeneration. Stages of regeneration included myoblastic proliferation, fusion into cords and myotubes, and, finally, fibrillogenesis to restore contractile material. The sequence of structural alterations in skeletal muscles of these affected pigs was discussed in terms of present theories of the biochemical role of these nutrients in maintaining cellular integrity.
对38头生长中的幼猪中的24头进行了光镜和电镜研究，这些猪喂食缺乏硒和维生素E的半合成饲料13至59天，其骨骼肌出现了病变。受损纤维的主要变化从透明变性开始，随后巨噬细胞侵入并吞噬破坏的肌浆，发展为通过成肌细胞增殖、融合并分化为具有成熟肌原纤维的纤维来进行肌纤维再生。最早的电镜变化是肌原纤维溶解和破坏，粗肌丝持续时间最长。线粒体、肌浆网和质膜的破坏发生在有肌原纤维改变的纤维中。肌膜的基膜在包绕的肌浆破坏后保留下来，并作为后续再生的支架。再生阶段包括成肌细胞增殖、融合成索和肌管，最后形成肌原纤维以恢复收缩物质。根据这些营养素在维持细胞完整性方面的生化作用的现有理论，讨论了这些受影响猪骨骼肌结构改变的顺序。
