用表达HIV-1和HPV 16抗原的戈登链球菌对食蟹猴进行阴道免疫。

Vaginal immunization of Cynomolgus monkeys with Streptococcus gordonii expressing HIV-1 and HPV 16 antigens.

作者信息

Di Fabio S, Medaglini D, Rush C M, Corrias F, Panzini G L, Pace M, Verani P, Pozzi G, Titti F

机构信息

Laboratorio di Virologia, Istituto Superiore di Sanità, Roma, Italy.

出版信息

Vaccine. 1998 Mar;16(5):485-92. doi: 10.1016/s0264-410x(97)80002-3.

DOI:10.1016/s0264-410x(97)80002-3

PMID:9491503

Abstract

Cynomolgus monkeys (Macaca fascicularis) were immunized by intravaginal administration of live recombinant Streptococcus gordonii. The vaccine strains of S. gordonii expressed the V3 domain of the gpl20 of human immunodeficiency virus type 1 (HIV-1), and the E7 protein of human papillomavirus type 16 (HPV 16). Multiple inocula of recombinant bacteria were used, since S. gordonii could persist for no longer than 3 days in the monkey vagina. Vaginal immunization was found to induce a local and systemic immune response specific for the heterologous antigen expressed by the bacteria. This antigen-specific immune response consisted of vaginal IgA, serum IgG, and a T-cell proliferative response measured on PBMCs. Vaginal IgG and serum IgA were not detected.

摘要

食蟹猴（猕猴）通过阴道内接种活的重组戈登链球菌进行免疫。戈登链球菌的疫苗菌株表达了1型人类免疫缺陷病毒（HIV-1）的gpl20的V3结构域和16型人乳头瘤病毒（HPV 16）的E7蛋白。由于戈登链球菌在猴阴道内持续时间不超过3天，因此使用了多次重组细菌接种。发现阴道免疫可诱导针对细菌表达的异源抗原的局部和全身免疫反应。这种抗原特异性免疫反应包括阴道IgA、血清IgG以及在PBMC上测得的T细胞增殖反应。未检测到阴道IgG和血清IgA。