Emmings F G, Evans R T, Genco R J
J Dent Res. 1976 Apr;55 Spec No:C181-92. doi: 10.1177/002203457605500312011.
M fascicularis monkeys were immunized subcutaneously in the vicinity of the major salivary glands and by retrograde infusion into the parotid duct, with a vaccine containing Formalin-killed S mutans strain 6715 cells and culture-fluid antigens. Indirect immunofluorescent staining was used to titrate and classify antibodies. Subcutaneous immunization induced only a serum response, whereas intraductal infusion stimulated both an IgA antibody response in the parotid fluid and a serum response. Immunized and nonimmunized control groups were orally infected with S mutans strain 6715. The establishment in dental plaque was quantitated by recovery of the infecting organism on selective media and by immunofluorescent staining of plaque smears taken from individual tooth surfaces. The establishment of S mutans strain 6715 was noticeably inhibited in immune monkeys. Immunofluorescent assays for antibody also showed that serum and parotid fluid containing serum IgA antibodies cross reacted with other d serotype and a serotype strains but not representative b and c strains. Immune and control groups were then orally infected with S mutans strain GS-5, a c serotype strain, and no inhibition in establishment was detected of the non-cross-reacting type c organism in the immune group. A latter series of booster immunizations via the intraductal route resulted in a significant decrease in parotid fluid flow. Histological investigations showed inflammatory cell infiltration and replacement of epithelium by connective tissue in the glands from immunized monkeys. A separate group of monkeys, younger than the first, was immunized with the same vaccine via the duct only. In this group, immunizations were given at shorter intervals, but the immunization response was similar to that observed in the first group. The investigations reviewed here and new experiments reported show that immunization of monkeys with S mutan strain 6715 via the parotid duct elicited a reproducible IgA antibody response in the parotid fluid as well as a serum antibody response. Inhibition of colonization on tooth surfaces in immune monkeys showed specificity for the immunizing strain suggesting that inhibition was antibody mediated.
将恒河猴在主要唾液腺附近进行皮下免疫，并通过逆行注入腮腺导管，使用含有福尔马林灭活的变形链球菌6715菌株细胞和培养液抗原的疫苗。采用间接免疫荧光染色法对抗体进行滴定和分类。皮下免疫仅诱导血清反应，而导管内注入则刺激腮腺液中的IgA抗体反应和血清反应。免疫组和未免疫的对照组经口感染变形链球菌6715菌株。通过在选择性培养基上回收感染菌以及对从单个牙齿表面采集的菌斑涂片进行免疫荧光染色来定量菌斑中的定植情况。在免疫的猴子中，变形链球菌6715菌株的定植明显受到抑制。抗体的免疫荧光测定还表明，含有血清IgA抗体的血清和腮腺液与其他d血清型和a血清型菌株发生交叉反应，但与代表性的b和c菌株不发生交叉反应。然后，免疫组和对照组经口感染c血清型菌株变形链球菌GS - 5，在免疫组中未检测到对非交叉反应性c型菌定植的抑制作用。随后通过导管途径进行的一系列加强免疫导致腮腺液流量显著减少。组织学研究显示，免疫猴子的腺体中有炎性细胞浸润以及结缔组织取代上皮。另一组比第一组年轻的猴子仅通过导管用相同疫苗进行免疫。在这组中，免疫间隔时间较短，但免疫反应与第一组观察到的相似。这里回顾的研究以及新报道的实验表明，通过腮腺导管用变形链球菌6715菌株免疫猴子可在腮腺液中引发可重复的IgA抗体反应以及血清抗体反应。免疫猴子牙齿表面定植的抑制对免疫菌株具有特异性，表明抑制是由抗体介导的。
