Eisenthal A, Marder O, Lifschitz-Mercer B, Skornick Y, Tirosh R, Irlin Y, Avtalion R, Deutsch M
Tel-Aviv Sourasky Medical Center, Israel.
Pathobiology. 1997;65(6):331-40. doi: 10.1159/000164144.
Natural killer (NK) cells play a role in the natural immunity against tumor cells. In the present study, we demonstrate that infection of the NK-sensitive tumor cell line K562 with influenza A virus caused a substantial increase in lysis of up to sevenfold when compared to noninfected cells. Similar to NK cells, IL-2-activated killer cells exhibited higher lytic activity against virus-infected K562 cells. This effect of the virus correlated with the increase in the expression of intracellular adhesion molecule-1 (ICAM-1) on K562 cells. Changes in the susceptibility to NK lysis were accompanied by alterations, within minutes, in the cytoskeleton as detected by intracellular fluorescein fluorescence polarization measured on the Cellscan, a static cytometer. The possible role of iCAM-1 and the cytoskeleton in the cytotoxic response of NK cells is discussed.
自然杀伤（NK）细胞在针对肿瘤细胞的天然免疫中发挥作用。在本研究中，我们证明，与未感染细胞相比，甲型流感病毒感染NK敏感的肿瘤细胞系K562导致细胞裂解大幅增加，高达七倍。与NK细胞类似，白细胞介素2激活的杀伤细胞对病毒感染的K562细胞表现出更高的裂解活性。病毒的这种作用与K562细胞上细胞间黏附分子1（ICAM-1）表达的增加相关。通过在静态细胞仪Cellscan上测量细胞内荧光素荧光偏振检测到，对NK裂解敏感性的变化在数分钟内伴随着细胞骨架的改变。本文讨论了ICAM-1和细胞骨架在NK细胞细胞毒性反应中的可能作用。
