Milbrandt J, de Sauvage F J, Fahrner T J, Baloh R H, Leitner M L, Tansey M G, Lampe P A, Heuckeroth R O, Kotzbauer P T, Simburger K S, Golden J P, Davies J A, Vejsada R, Kato A C, Hynes M, Sherman D, Nishimura M, Wang L C, Vandlen R, Moffat B, Klein R D, Poulsen K, Gray C, Garces A, Johnson E M
Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Neuron. 1998 Feb;20(2):245-53. doi: 10.1016/s0896-6273(00)80453-5.
A novel neurotrophic factor named Persephin that is approximately 40% identical to glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) has been identified using degenerate PCR. Persephin, like GDNF and NTN, promotes the survival of ventral midbrain dopaminergic neurons in culture and prevents their degeneration after 6-hydroxydopamine treatment in vivo. Persephin also supports the survival of motor neurons in culture and in vivo after sciatic nerve axotomy and, like GDNF, promotes ureteric bud branching. However, in contrast to GDNF and NTN, persephin does not support any of the peripheral neurons that were examined. Fibroblasts transfected with Ret and one of the coreceptors GFRalpha-1 or GFRalpha-2 do not respond to persephin, suggesting that persephin utilizes additional, or different, receptor components than GDNF and NTN.
利用简并PCR已鉴定出一种名为Persephin的新型神经营养因子，它与胶质细胞系源性神经营养因子（GDNF）和神经营养素（NTN）约有40%的同源性。与GDNF和NTN一样，Persephin能促进培养的腹侧中脑多巴胺能神经元的存活，并在体内6-羟基多巴胺处理后防止其退化。Persephin还能支持坐骨神经切断术后培养的和体内运动神经元的存活，并且与GDNF一样，能促进输尿管芽分支。然而，与GDNF和NTN不同的是，Persephin不支持所检测的任何外周神经元。用Ret和共受体之一GFRalpha-1或GFRalpha-2转染的成纤维细胞对Persephin无反应，这表明Persephin利用的受体成分与GDNF和NTN不同或有所增加。
