Palmer M V, Elsasser T H, Cheville N F
Zoonotic Disease Research Unit, ARS, USDA, Ames, IA 50010, USA.
Am J Vet Res. 1998 Feb;59(2):153-6.
To determine the influence of brucellosis vaccination on tumor necrosis factor-alpha (TNF-alpha) concentrations in pregnant cattle and the possible role of the bovine placenta in TNF-alpha production.
Polled Hereford heifers obtained from a nonvaccinated, brucellosis-free herd and bred at 16 to 27 months at age. All cattle were seronegative for Brucella abortus by results of the standard tube agglutination test.
At 6 months' gestation, cattle were vaccinated i.v. with B abortus strain RB51 (n = 10), s.c. with B abortus strain RB51 (n = 5), or s.c. with B abortus strain 19 (n = 5); controls received pyrogen-free saline solution s.c. (n = 2). Blood samples were collected periodically for TNF-alpha assays. At necropsy, 8 to 12 weeks after vaccination, placental fluids and fetal blood were collected for TNF-alpha analysis and placental tissues were collected for immunohistochemical detection of TNF-alpha.
Radioimmunoassays indicated no increase in TNF-alpha concentration in blood from i.v. or s.c. vaccinated cattle, compared with controls. Similarly, TNF-alpha concentrations in amniotic and allantoic fluids from s.c. vaccinated cattle were not different from values for controls. Although only i.v. vaccinated cattle developed placentitis, immunohistochemical analysis for TNF-alpha revealed increased immunoreactivity within placental trophoblastic epithelial cells of s.c. and i.v. vaccinated cattle.
s.c. vaccination for prevention of brucellosis, using recommended adult dosages, does not result in increase of TNF-alpha concentration in plasma, serum, or placental fluids; however, vaccination of pregnant cattle stimulates trophoblastic epithelial cells to express TNF-alpha, although the physiologic and quantitative importance of this expression remains unknown.
确定布鲁氏菌病疫苗接种对妊娠母牛肿瘤坏死因子-α（TNF-α）浓度的影响以及牛胎盘在TNF-α产生中的可能作用。
从一个未接种疫苗、无布鲁氏菌病的牛群中选取无角海福特小母牛，在16至27月龄时进行配种。通过标准试管凝集试验结果，所有牛的布鲁氏菌流产亚种血清学检测均为阴性。
妊娠6个月时，给牛静脉注射RB51株布鲁氏菌流产亚种（n = 10）、皮下注射RB51株布鲁氏菌流产亚种（n = 5）或皮下注射19株布鲁氏菌流产亚种（n = 5）；对照组皮下注射无热原生理盐水（n = 2）。定期采集血样进行TNF-α检测。在接种疫苗8至12周后进行尸检，收集胎盘液和胎儿血液进行TNF-α分析，并收集胎盘组织进行TNF-α的免疫组织化学检测。
放射免疫分析表明，与对照组相比，静脉注射或皮下注射疫苗的牛血液中TNF-α浓度没有增加。同样，皮下注射疫苗的牛羊水和尿囊液中TNF-α浓度与对照组的值没有差异。虽然只有静脉注射疫苗的牛发生了胎盘炎，但对TNF-α的免疫组织化学分析显示，皮下注射和静脉注射疫苗的牛胎盘滋养层上皮细胞内的免疫反应性增加。
使用推荐的成人剂量进行皮下接种预防布鲁氏菌病，不会导致血浆、血清或胎盘液中TNF-α浓度升高；然而，妊娠母牛接种疫苗会刺激滋养层上皮细胞表达TNF-α，尽管这种表达的生理和定量重要性尚不清楚。
