Nikonorov Iu M, Chemeris A V, Vil'danov I M, Vakhitov V A
Department of Biochemistry and Cytochemistry, Ufa Research Center, Russian Academy of Sciences, Russia.
Genetika. 1997 Dec;33(12):1649-54.
In diploid wheat Triticum monococcum L., a novel family of AT-rich repeated DNA sequences arranged in clusters of tandem arrays of 560-bp fragments is described. The nucleotide sequence of a 556-bp repeat unit was determined. The basic structural unit of this repeated DNA family contains subrepeats with the main motif AAACATTGTTAC. This type of repetitive DNA sequence could evolve via tandem amplification of short subrepeats with some subsequent divergence, and/or via recombination with unique sequences. Structural organization of homologous sequences, revealed by blot hybridization in other representatives of the Triticeae tribe, suggest that this family of repeated DNA is genome-specific.
在二倍体小麦一粒小麦(Triticum monococcum L.)中,描述了一个新的富含AT的重复DNA序列家族,这些序列以560 bp片段的串联阵列簇形式排列。确定了一个556 bp重复单元的核苷酸序列。这个重复DNA家族的基本结构单元包含以AAACATTGTTAC为主基序的亚重复序列。这种类型的重复DNA序列可能通过短亚重复序列的串联扩增以及随后的一些分化,和/或通过与独特序列的重组而进化。通过对小麦族其他代表物种的印迹杂交揭示的同源序列的结构组织表明,这个重复DNA家族是基因组特异性的。