Menuzo's B2培养基添加水牛大鼠肝细胞对体外成熟/体外受精牛卵母细胞发育的有效性。
Effectiveness of Menuzo's B2 medium with buffalo rat liver cells for development of in vitro matured/in vitro fertilized bovine oocytes.
作者信息
Krisher R L, Gibbons J R, Gwazdauskas F C
机构信息
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg 24061-0315, USA.
出版信息
J Assist Reprod Genet. 1998 Jan;15(1):50-3. doi: 10.1023/a:1022582422932.
PURPOSE
The effectiveness of two culture media on the development of bovine embryos in a buffalo rat liver (BRL) coculture system was investigated.
METHODS
Bovine oocytes were matured and fertilized in vitro, then cocultured, 25 per well, for 7 days in 500 microliters of modified M199 or modified Menuzo's B2 medium over a BRL cell monolayer at 39 degrees C in an atmosphere of 5% CO2 in air. Medium 199 was modified by the addition of 10% of (v/v) fetal bovine serum (FBS), 9 mg/ml bovine serum albumin, 2 mM glycine, 1 mM alanine, and 0.1 mM nonessential amino acids (NEAA). Menuzo's B2 medium was modified by the addition of 10% (v/v) FBS, 1 mM alanine, and 0.1 mM NEAA.
RESULTS
Modified Menuzo's B2 medium improved embryo development to the morula or blastocyst stage compared to modified M199 (121/353, 34.3%, versus 99/362, 27.3%, P < 0.05).
CONCLUSIONS
These results suggest that Menuzo's B2 medium with modifications in a BRL coculture system can provide a significant benefit for culture of early bovine embryos over the traditional use of medium 199.
目的
研究两种培养基在水牛大鼠肝脏（BRL）共培养系统中对牛胚胎发育的有效性。
方法
牛卵母细胞在体外成熟并受精，然后以每孔25个的密度，在39℃、含5%二氧化碳的空气环境中，于500微升改良M199或改良的Ménézo's B2培养基中，在BRL细胞单层上共培养7天。改良M199培养基添加了10%（体积/体积）胎牛血清（FBS）、9毫克/毫升牛血清白蛋白、2毫摩尔/升甘氨酸、1毫摩尔/升丙氨酸和0.1毫摩尔/升非必需氨基酸（NEAA）。改良的Ménézo's B2培养基添加了10%（体积/体积）FBS、1毫摩尔/升丙氨酸和0.1毫摩尔/升NEAA。
结果
与改良M199相比，改良的Ménézo's B2培养基使胚胎发育至桑椹胚或囊胚阶段的比例提高（121/353，34.3%，对比99/362，27.3%，P<0.05）。
结论
这些结果表明，在BRL共培养系统中经改良的Ménézo's B2培养基，相较于传统使用的M199培养基，能为牛早期胚胎培养带来显著益处。
Zotero 插件