利用偏振拉曼技术研究聚-L-赖氨酸在甲醇水溶液中的构象
Study of poly-L-lysine conformations in aqueous methanol solution by using polarized Raman techniques.
作者信息
Shepherd I W
出版信息
Biochem J. 1976 Jun 1;155(3):543-8. doi: 10.1042/bj1550543.
Abstract
Raman polarization measurements of the amide I band are reported in ionized poly-L-lysine dissolved in aqueous methanol. The observed changes with methanol concentration, attributed to changes in coil conformation and to the helix-coil transition, represent a novel method of measuring polymer conformation. Polarization measurements as a function of temperature yield values of the energy differences between rotational isomeric states in the coil. deltaH, of 8.8 +/- 0.7, 10.4 +/- 1.1 and 10.8 +/- 1.5 kJ/mol at methanol concentrations (v/v) of 85, 80 and 70% respectively. The stabilization energy of the helix is estimated at 9.3 kJ/mol.
摘要
报道了在溶解于甲醇水溶液中的离子化聚-L-赖氨酸中酰胺I带的拉曼偏振测量结果。观察到的随甲醇浓度的变化，归因于线圈构象的变化和螺旋-线圈转变，代表了一种测量聚合物构象的新方法。作为温度函数的偏振测量给出了线圈中旋转异构体状态之间能量差的值。在甲醇浓度（v/v）分别为85%、80%和70%时，ΔH分别为8.8±0.7、10.4±1.1和10.8±1.5 kJ/mol。螺旋的稳定能估计为9.3 kJ/mol。
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