Klump H
Biophys Chem. 1976 Sep;5(3):363-7. doi: 10.1016/0301-4622(76)80048-8.
The transition enthalpy deltaH of the helix-random coil transition of the DNA-polylysine complex was measured as a function of the peptide:nucleotide ratio by the help of an adiabatic scanning differential calorimeter. Furthermore the transition enthalpy of a complex with a specific peptide:nucleotide ratio was determined as a function of the cation concentration of the solution. Finally the reaction enthalpy of the interaction of polylysine with native and denatured DNA was measured with the help of a LKB batch calorimeter. From the results of the calorimetric measurements one can conclude that the transition enthalpy of the DNA-polylysine complexes is linearly dependent on the nucleotide: peptide ratio. The extrapolated value for the 1:1 complex is 14.4 kcal per mole base pairs.
借助绝热扫描差示量热计，测量了DNA - 聚赖氨酸复合物螺旋 - 无规卷曲转变的转变焓ΔH随肽与核苷酸比例的变化。此外，还测定了具有特定肽与核苷酸比例的复合物的转变焓随溶液阳离子浓度的变化。最后，借助LKB批量量热计测量了聚赖氨酸与天然和变性DNA相互作用的反应焓。从量热测量结果可以得出结论，DNA - 聚赖氨酸复合物的转变焓与核苷酸：肽比例呈线性相关。1:1复合物的外推值为每摩尔碱基对14.4千卡。
