离体灌注的泌乳豚鼠乳腺对L-(U-¹⁴C)缬氨酸、L-(U-¹⁴C)亮氨酸、L-(U-¹⁴C)组氨酸和L-(U-¹⁴C)苯丙氨酸的代谢
Metabolism of L-(U-14C)valine, L-(U-14C)leucine, L-(U-14C)histidine and L-(U-14C) phenylalanine by the isolated perfused lactating guinea-pig mammary gland.
作者信息
Davis S R, Mepham T B
出版信息
Biochem J. 1976 Jun 15;156(3):553-60. doi: 10.1042/bj1560553.
Abstract
- The incorporation of L-[U-14C]leucine, L[U-14C]histidine and L-[U-14C]phenylalanine into casein secreted during perfusion of isolated guinea-pig mammary glands was demonstrated. 2. The extent of incorporation of label into casein residues was consistent with their being derived from free amino acids of the perfusate plasma. 3. The mean transit time of the amino acids from perfusate into secreted casein was approx. 100 min. 4. Whereas radioactive histidine and phenylalanine were incorporated solely into milk protein, radioactivity from [U-14C]valine was also transferred to CO2 and to an unidentified plasma component, and from [U-14C]leucine to plasma glutamic acid. 5. Evidence from experiments with [U-14C]phenylalanine suggests that, as in rats, but in contrast with ruminant species, guinea-pig mammary tissue does not possess phenyl alanine hydroxylase activity. 6. The results are discussed in relation to the possible role of essential amino acid catabolism in the control of milk-protein synthesis.
摘要
- 已证实，在对分离的豚鼠乳腺进行灌注期间，L-[U-14C]亮氨酸、L-[U-14C]组氨酸和L-[U-14C]苯丙氨酸掺入所分泌的酪蛋白中。2. 标记物掺入酪蛋白残基的程度与其来源于灌注液血浆中的游离氨基酸一致。3. 氨基酸从灌注液进入分泌的酪蛋白的平均转运时间约为100分钟。4. 放射性组氨酸和苯丙氨酸仅掺入乳蛋白中，而[U-14C]缬氨酸的放射性也转移至二氧化碳和一种未鉴定的血浆成分，[U-14C]亮氨酸的放射性则转移至血浆谷氨酸中。5. 用[U-14C]苯丙氨酸进行实验的证据表明，与大鼠一样，但与反刍动物不同，豚鼠乳腺组织不具有苯丙氨酸羟化酶活性。6. 讨论了这些结果与必需氨基酸分解代谢在控制乳蛋白合成中可能作用的关系。
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