一种基于测量蛋白质中2-³H标记氨基酸来测定蛋白质周转的灵敏方法。
A sensitive method for measuring protein turnover based on the measurement of 2-3H-labelled amino acids in protein.
作者信息
Humphrey T J, Davies D D
出版信息
Biochem J. 1976 Jun 15;156(3):561-8. doi: 10.1042/bj1560561.
Abstract
A method for measuring the rate of protein degradation is described. The method measures the change in 2-3H content of protein with time by racemization of the protein hydrolysate with acetic anhydride. The 3H on C-2 of amino acids is stable in proteins but becomes labile, owing to the action of transaminases, once the amino acids are released by proteolysis. The specific measurement of 2-3H in amino acids largely overcomes problems due to compartmentation and isotope recycling and evidence to support this claim is presented. Values for the half-life of Lemna minor (duckweed) protein determined by the new method are compared with values obtained by other methods.
摘要
本文描述了一种测量蛋白质降解速率的方法。该方法通过用乙酸酐使蛋白质水解产物消旋来测量蛋白质中2-³H含量随时间的变化。氨基酸C-2位上的³H在蛋白质中是稳定的，但一旦氨基酸通过蛋白水解作用释放出来，由于转氨酶的作用，它就会变得不稳定。对氨基酸中2-³H的特异性测量在很大程度上克服了由于区室化和同位素循环导致的问题，并提供了支持这一说法的证据。将通过新方法测定的浮萍蛋白半衰期值与通过其他方法获得的值进行了比较。
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本文引用的文献
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The Turnover of Nucleic Acids in Lemna minor.浮萍中核酸的周转
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2
Protein metabolism in cultured plant tissues. Calculation of an absolute rate of protein synthesis, accumulation, and degradation in tobacco callus in vivo.培养植物组织中的蛋白质代谢。体内烟草愈伤组织中蛋白质合成、积累和降解绝对速率的计算。
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D2O-alanine exchange reactions catalyzed by alanine racemase and glutamic pyruvic transminase.由丙氨酸消旋酶和谷丙转氨酶催化的重水-丙氨酸交换反应。
Biochem Biophys Res Commun. 1974 May 20;58(2):460-6. doi: 10.1016/0006-291x(74)90387-8.
4
A new method for the measurement of protein turnover.一种测量蛋白质周转率的新方法。
Biochem J. 1975 Apr;148(1):119-27. doi: 10.1042/bj1480119.
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