体内蛋白质周转率测量方法的比较。
A comparison of methods for the measurement of protein turnover in vivo.
作者信息
MacDonald M L, Augustine S L, Burk T L, Swick R W
出版信息
Biochem J. 1979 Nov 15;184(2):473-6. doi: 10.1042/bj1840473.
Abstract
Steady-state rates of turnover of two single proteins were measured in vivo by two independent methods. The fractional rate of synthesis of liver ornithine aminotransferase, measured by a continuous infusion of L-[2,6-3H]tyrosine, was 0.42 day-1, whereas in the same animals the fractional rate of degradation measured by loss of radioactivity from amino acids labelled via [14C]bicarbonate was 0.40 day-1. The agreement between methods confirms the reliability of each method for the study of hepatic protein turnover. In contrast, [14C]bicarbonate-labelled amino acids are extensively reutilized in muscle, and are therefore unsuitable for measuring rates of muscle protein breakdown.
摘要
通过两种独立的方法在体内测量了两种单一蛋白质的稳态周转率。通过连续输注L-[2,6-³H]酪氨酸测量肝脏鸟氨酸转氨酶的合成分数率为0.42天⁻¹，而在相同动物中，通过[¹⁴C]碳酸氢盐标记的氨基酸放射性损失测量的降解分数率为0.40天⁻¹。两种方法之间的一致性证实了每种方法用于研究肝脏蛋白质周转的可靠性。相比之下，[¹⁴C]碳酸氢盐标记的氨基酸在肌肉中被大量再利用，因此不适合用于测量肌肉蛋白质分解率。
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