红细胞钙代谢。正常及镰状细胞贫血红细胞中的钙交换
Erythrocyte calcium metabolism. Calcium exchange in normal and sickle-cell-anaemia erythrocytes.
作者信息
Cameron B F, Smariga P E
出版信息
Biochem J. 1976 Jun 15;156(3):577-83. doi: 10.1042/bj1560577.
Under exchange conditions (no net increase in calcium), erythrocytes incubated in isoosmotic phosphate-buffered saline have an exchangeable calcium pool comprising about 10% of the total erythrocyte calcium. This pool reaches exchange equilibrium, for either inward-directed or outward-directed transfer of the 45Ca-exchange label, with a half-time of about 20 min. The uptake of Ca2+ requires phosphate, even under hypo-osmotic conditions, where the calcium loading expected as the cells swell is obtained only when phosphate is present. The phosphate requirement is not due to Ca2+ transport as a phosphate salt. This exchangeable-calcium pool is also present in sickle-cell-anemia erythrocytes, and comprises a similar proportion of total cellular calcium.
在交换条件下（钙无净增加），在等渗磷酸盐缓冲盐溶液中孵育的红细胞具有一个可交换钙池，其钙含量约占红细胞总钙含量的10%。对于45Ca交换标记的内向或外向转移，这个池达到交换平衡的半衰期约为20分钟。即使在低渗条件下，Ca2+的摄取也需要磷酸盐，只有当存在磷酸盐时，随着细胞肿胀预期的钙负载才能实现。对磷酸盐的需求并非源于作为磷酸盐盐的Ca2+运输。这个可交换钙池也存在于镰状细胞贫血红细胞中，并且在细胞总钙中占类似比例。
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