Rabinowitz I N, Wolf P L, Berman S, Shikuma N, Edwards P
Prostaglandins. 1975 Apr;9(4):545-55. doi: 10.1016/0090-6980(75)90060-x.
Prostaglandin E2 has previously been shown to enhance the shape transformation of sickle prone erythrocytes (8) and to reduce the oxygen resaturation of Hemoglobin SS within intact sickle cell erythrocytes after deoxygenation (15). In view of the recent importance attributed to calcium transport in maintaining erythrocyte shape and viability (10) and the suggestion that prostaglandins may act via a calcium ionophore mechanism (9) on cell membranes, erythrocyte ghosts were prepared following the method of Lepke and Passow (12) from normal and sickle cell anemia erythrocytes. These two classes of ghosts are shown to display differing patterns of sodium and calcium transport, whith calcium influx being preferentially stimulated by prostaglandin E2 in sickle cell ghosts. It is suggested that in hypoxic, stasis conditions in vivo, prostaglandins may play a role in accelerating sickling of sickle prone erythrocytes via stimulation of calcium influx.
前列腺素E2此前已被证明可增强镰状易变红细胞的形态转化(8)，并在脱氧后降低完整镰状细胞红细胞内血红蛋白SS的氧再饱和(15)。鉴于最近钙转运在维持红细胞形态和活力方面的重要性(10)，以及前列腺素可能通过钙离子载体机制(9)作用于细胞膜的观点，按照Lepke和Passow(12)的方法从正常和镰状细胞贫血红细胞中制备了红细胞影。结果表明，这两类红细胞影表现出不同的钠和钙转运模式，在镰状细胞影中，前列腺素E2优先刺激钙内流。有人提出，在体内缺氧、血流停滞的情况下，前列腺素可能通过刺激钙内流在加速镰状易变红细胞的镰变过程中发挥作用。
