Vengerova T I, Petrosian M N, Bogacheva G T, Rokhlin O V
Mol Biol (Mosk). 1979 Nov-Dec;13(6):1303-11.
Monospecific antibodies against V-region of the L-chain of the mouse myeloma protein MOPC21 (VK 15 group) were obtained and used for preparation of antibody-immunoadsorbents. By means of the immunoadsorbents the content of normal immunoglobulins with antigenic markers of VK 15 group in the serum of mouse strains BALB/c, CBA and C57BL/6 was determined. The least quantity of the proteins determined by this method was estimated to be 1.4%. If each of K-chains of the variable groups is represented equally in the population of normal immunoglobulins, the number of VK groups and respectively germ-line VK genes should be not less than 70.
获得了针对小鼠骨髓瘤蛋白MOPC21（VK 15组）轻链V区的单特异性抗体，并将其用于制备抗体免疫吸附剂。借助这些免疫吸附剂，测定了BALB/c、CBA和C57BL/6小鼠品系血清中具有VK 15组抗原标记的正常免疫球蛋白的含量。用这种方法测定的蛋白质的最小量估计为1.4%。如果可变组的每个K链在正常免疫球蛋白群体中均等存在，那么VK组以及相应的种系VK基因的数量应不少于70。
