Pernet P, Aussel C, Le Boucher J, Giboudeau J, Cynober L, Coudray-Lucas C
Service de Biochimie A, Hôpital Saint-Antoine, GRENEMH, CHU Saint-Antoine, Paris, France.
Eur Surg Res. 1998;30(1):69-76. doi: 10.1159/000008560.
Intestinal permeability can be assessed by measuring urinary mannitol and lactulose excretion after oral administration. This test may be useful as a tool in experimental studies to explore the effects of specific diets intended to promote the repair of the integrity of the gut barrier. In this study we standardized the lactulose-mannitol test in rats and applied it to a burned-rat model. The conditions were: oral administration of an isotonic mixture of 50 mg of mannitol and 66 mg of lactulose, followed by aseptic collection of urine over 4 h. The increase in the lactulose/mannitol ratio in burned rats was due to higher lactulose excretion. These results corroborate those obtained in burn patients and show that our model is suitable for further experiments on nutritional manipulation.
肠道通透性可通过口服后测量尿中甘露醇和乳果糖排泄量来评估。该测试作为一种工具，在实验研究中可能有助于探索旨在促进肠道屏障完整性修复的特定饮食的效果。在本研究中，我们对大鼠的乳果糖 - 甘露醇测试进行了标准化，并将其应用于烧伤大鼠模型。条件为：口服50毫克甘露醇和66毫克乳果糖的等渗混合物，随后在4小时内无菌收集尿液。烧伤大鼠中乳果糖/甘露醇比值的增加是由于乳果糖排泄量增加。这些结果证实了在烧伤患者中获得的结果，并表明我们的模型适用于进一步的营养调控实验。
