Leonard J T, Grace M B, Buzard G S, Mullen M J, Barbagallo C B
Millipore, Danvers, MA, USA.
Biotechniques. 1998 Feb;24(2):314-7. doi: 10.2144/98242pf01.
We demonstrate that routine PCR product analytical agarose gels can also serve as preparative gels for quick DNA template purification before sequencing. The band of interest is excised, placed into a Gel Nebulizer inside a Micropure separator and rapidly purified in a single centrifugation step. Gel-purified PCR product, suitable for manual and automated sequencing, is delivered within 10 min.
我们证明，常规PCR产物分析用琼脂糖凝胶也可作为制备凝胶，用于测序前快速纯化DNA模板。将感兴趣的条带切下，放入Micropure分离器内的凝胶雾化器中，通过单步离心快速纯化。凝胶纯化的PCR产物适用于手动和自动测序，10分钟内即可完成。
