Poyer J F, Chan K Y, Arshinoff S A
Alcon Laboratories, Inc., Fort Worth, Texas 76134-2099, USA.
J Cataract Refract Surg. 1998 Jan;24(1):84-90. doi: 10.1016/s0886-3350(98)80079-5.
To quantify the surface retention of several ophthalmic viscoelastic agents following irrigation and aspiration (I/A) using a new in vitro method.
Alcon Laboratories, Inc., Fort Worth, Texas, USA.
A rabbit corneal endothelial cell line was cultured to confluency in 24-well plates, and the cells were labeled quantitatively with internalized neutral red dye. Five ophthalmic viscoelastic agents were applied to cover the monolayer of cells: sodium hyaluronate (Healon, Provisc, and Amvisc Plus), Formulation A (a dispersive, nonproteinaceous, synthetic polymer), and sodium chondroitin sulfate, sodium hyaluronate (Viscoat). Irrigation and aspiration (with fluid turbulence similar to that encountered in phacoemulsification surgery) were performed on each well for 3 minutes, using 120 mL of balanced salt solution with bicarbonate. dextrose, and glutathione (BSS Plus). The cells were treated with an acidified ethanol solution to extract the dye from the cells left without a viscoelastic cover. The extracted dye was measured by spectrophotometry and compared with the total dye recovered from control cells.
The retention value, which represented the percentage of cells with viscoelastic retained on the surface, was calculated as follows: Healon, 7; Provisc, 16; Amvisc Plus, 17; Formulation A, 55; Viscoat, 90. On a nonadsorptive, non-cell surface, the retention values of the five viscoelastics were significantly less than those on cells.
The results of this experimental model suggest that cohesive viscoelastics are readily removed from the cells, while dispersive viscoelastics are highly retained. In addition, mutual surface interaction (electrical charge and other properties) plays a significant role in determining the retention of viscoelastics on the corneal endothelial cell surface following I/A.
使用一种新的体外方法，对几种眼科粘弹性剂在冲洗和抽吸（I/A）后的表面残留量进行定量分析。
美国得克萨斯州沃思堡市爱尔康实验室公司。
将兔角膜内皮细胞系培养至铺满24孔板，并用内化中性红染料对细胞进行定量标记。应用五种眼科粘弹性剂覆盖细胞单层：透明质酸钠（Healon、Provisc和Amvisc Plus）、配方A（一种分散性、非蛋白质合成聚合物）以及硫酸软骨素钠、透明质酸钠（Viscoat）。对每孔使用120 mL含碳酸氢盐、葡萄糖和谷胱甘肽的平衡盐溶液（BSS Plus）进行冲洗和抽吸（流体湍流类似于超声乳化手术中遇到的情况），持续3分钟。用酸化乙醇溶液处理细胞，以从没有粘弹性覆盖的细胞中提取染料。通过分光光度法测量提取的染料，并与从对照细胞中回收的总染料进行比较。
残留值代表表面保留有粘弹性剂的细胞百分比，计算如下：Healon为7%；Provisc为16%；Amvisc Plus为17%；配方A为55%；Viscoat为90%。在非吸附性、非细胞表面，五种粘弹性剂的残留值明显低于在细胞上的残留值。
该实验模型的结果表明，粘性粘弹性剂很容易从细胞上清除，而分散性粘弹性剂则高度残留。此外，相互表面相互作用（电荷和其他性质）在决定I/A后粘弹性剂在角膜内皮细胞表面的残留方面起着重要作用。
