Kadowaki H, Israel H W, Nesheim M C
Biochim Biophys Acta. 1976 Jun 23;437(1):158-65. doi: 10.1016/0304-4165(76)90357-3.
Although chickens are uricotelic and do not have significant urea-ornithine cycle in any tissue, the kidneys contain a high concentration of arginase which apparently functions to regulate degradation of dietary arginine. A series of investigations has been made to determine the intracellular localization of this arginase in chicken kidney. Tissue fractionation using sucrose density gradients and differential centrifugation showed as association of arginase activity with certain marker enzymes and with fractions identified as mitochondria by electron microscopy. This is consistent with the localization of the arginase in the mitochondrial matrix of chicken kidney cells. Such a finding has significance in understanding the regulation of arginine degradation in chickens.
虽然鸡是排尿酸动物，且任何组织中都没有显著的尿素-鸟氨酸循环，但肾脏中含有高浓度的精氨酸酶，其作用显然是调节日粮精氨酸的降解。已进行了一系列研究以确定该精氨酸酶在鸡肾脏中的细胞内定位。使用蔗糖密度梯度和差速离心进行的组织分级分离显示，精氨酸酶活性与某些标记酶以及通过电子显微镜鉴定为线粒体的组分相关。这与精氨酸酶在鸡肾细胞线粒体基质中的定位一致。这一发现对于理解鸡体内精氨酸降解的调节具有重要意义。
