Wang X L, Uzawa K, Miyakawa A, Shiiba M, Watanabe T, Sato T, Miya T, Yokoe H, Tanzawa H
Department of Oral Surgery, Chiba University School of Medicine, Japan.
Int J Cancer. 1998 Mar 2;75(5):671-4. doi: 10.1002/(sici)1097-0215(19980302)75:5<671::aid-ijc2>3.0.co;2-w.
To search for the existence of a tumour-suppressor gene (TSG) associated with oral squamous cell carcinoma (SCC), PCR analysis of microsatellite polymorphisms corresponding to 14 loci which map to chromosome 7q21.3-qter was performed to screen 35 patients with oral SCC for loss of heterozygosity (LOH). LOH was observed in at least one of the loci in 19 of 34 (55.9%) informative cases. Among the loci tested, frequent LOH was restricted at D7S522 on chromosome 7q31.1, which was measured within 1 cM. Furthermore, we detected microsatellite instability (MI) in 11 of 35 (31.4%) cases tested. Our observations indicate that alterations of chromosome 7q are associated with oral SCC tumorigenesis and that 7q31.1 might harbour at least one putative TSG.
为了寻找与口腔鳞状细胞癌（SCC）相关的肿瘤抑制基因（TSG），我们对位于7号染色体7q21.3 - qter区域的14个位点的微卫星多态性进行了聚合酶链反应（PCR）分析，以筛查35例口腔SCC患者的杂合性缺失（LOH）情况。在34例（55.9%）可提供信息的病例中，有19例在至少一个位点观察到LOH。在所检测的位点中，频繁的LOH局限于7号染色体7q31.1上的D7S522，该位点位于1厘摩（cM）范围内。此外，在35例检测病例中有11例（31.4%）检测到微卫星不稳定性（MI）。我们的观察结果表明，7号染色体q臂的改变与口腔SCC的肿瘤发生相关，并且7q31.1可能至少含有一个假定的TSG。
