Kondo Y, Mizokami M, Nakano T, Kato T, Ohba K, Orito E, Ueda R, Mukaide M, Hikiji K, Oyunsuren T, Cooksley W G
Second Department of Medicine, Nagoya City University Medical School, Mizuho, Nagoya, Japan.
Virus Res. 1997 Dec;52(2):221-30. doi: 10.1016/s0168-1702(97)00123-8.
GB virus C/hepatitis G virus is a newly described virus. Classification of GB virus C/hepatitis G virus into genotypes has not been established. We analyzed nucleotide sequences within the 5' untranslated region of GB virus C/hepatitis G virus isolates and segregated these isolates into genotypes. Twenty serum samples with GB virus C/hepatitis G virus RNA from Australia, Cameroon, the Congo, Japan, Mongolia, and Bangladesh were studied. Reverse transcription and polymerase chain reaction were used to obtain GB virus C/hepatitis G virus RNA. After nucleotide sequences from the 5' untranslated region were determined, 68 nucleotide sequences, including 48 previously reported sequences, were analyzed by molecular evolutionary methods. The phylogenetic tree of the 5' untranslated region showed that all strains could be divided into three major genotypes, GB type (type 1), HG type (type 2), and Asian type (type 3). Bootstrap analysis indicated that the strains could be divided into three major genotypes but could not be further subdivided. Moreover, frequency histograms of pairwise distances between nucleotide sequences demonstrated only one peak. These result indicated that GB virus C/hepatitis G virus can be classified into three major genotypes, GB type (type 1), HG type (type 2), and Asian type (type 3), and should not be divided into minor subtypes.
庚型肝炎病毒/GB病毒C是一种新发现的病毒。庚型肝炎病毒/GB病毒C的基因型分类尚未确定。我们分析了庚型肝炎病毒/GB病毒C分离株5'非编码区的核苷酸序列，并将这些分离株分为不同的基因型。对来自澳大利亚、喀麦隆、刚果、日本、蒙古和孟加拉国的20份含有庚型肝炎病毒/GB病毒C RNA的血清样本进行了研究。采用逆转录和聚合酶链反应获取庚型肝炎病毒/GB病毒C RNA。测定5'非编码区的核苷酸序列后，运用分子进化方法分析了68个核苷酸序列，其中包括48个先前报道的序列。5'非编码区的系统发育树表明，所有毒株可分为三个主要基因型，即GB型（1型）、HG型（2型）和亚洲型（3型）。自展分析表明，这些毒株可分为三个主要基因型，但无法进一步细分。此外，核苷酸序列间成对距离的频率直方图仅显示一个峰值。这些结果表明，庚型肝炎病毒/GB病毒C可分为三个主要基因型，即GB型（1型）、HG型（2型）和亚洲型（3型），不应再分为更小的亚型。
