Li G, Shinozuka J, Uetsuka K, Nakayama H, Doi K
Department of Veterinary Pathology, Faculty of Agriculture, The University of Tokyo, Japan.
Exp Toxicol Pathol. 1997 Dec;49(6):447-50. doi: 10.1016/S0940-2993(97)80132-7.
The characteristics of T-2 toxin-induced cell damage in the intestinal crypt epithelia was investigated in mice. Following T-2 toxin-inoculation (0, 2.5, 5 and 10 mg/kg b.w.), dead cells showing pyknosis were sporadically observed in the crypt epithelia, and the nuclei of these cells were strongly stained by the modified TUNEL method which detects fragmented DNA in situ. Electron microscopically, the dead cells were characterized by shrinkage of the cell body and condensation of nuclear chromatin frequently along the nuclear membrane, and such nuclei were sometimes fragmented into small pieces. These morphological characteristics are well consistent with those of apoptosis. The mitotic index in the crypt epithelia drastically decreased at 6 hours after T-2 toxin-inoculation (6 HAI), but thereafter it recovered to almost the same value with that in control mice at 48 HAI. On the other hand, the apoptotic index in the crypt epithelia increased with the lapse of time. Clear mouse strain- and sex-differences were detected in the apoptotic index but not in the mitotic index. This is the first report that T-2 toxin caused apoptotic cell death in the intestinal crypt epithelial cells.
研究了T-2毒素诱导小鼠肠隐窝上皮细胞损伤的特征。在接种T-2毒素（0、2.5、5和10mg/kg体重）后，在隐窝上皮中偶尔观察到显示核固缩的死亡细胞，并且通过检测原位DNA片段化的改良TUNEL方法对这些细胞的细胞核进行了强染色。电子显微镜下，死亡细胞的特征是细胞体收缩和核染色质经常沿核膜凝聚，并且这样的细胞核有时会破碎成小碎片。这些形态学特征与细胞凋亡的特征非常一致。T-2毒素接种后6小时（HAI），隐窝上皮中的有丝分裂指数急剧下降，但此后在48 HAI时恢复到与对照小鼠几乎相同的值。另一方面，隐窝上皮中的凋亡指数随时间增加。在凋亡指数中检测到明显的小鼠品系和性别差异，但在有丝分裂指数中未检测到。这是关于T-2毒素导致肠隐窝上皮细胞凋亡性细胞死亡的首次报道。
