Wichelhaus T A, Schulze J, Hunfeld K P, Schäfer V, Brade V
Department of Medical Microbiology, University Hospital of Frankfurt, Frankfurt am Main, Germany.
Eur J Clin Microbiol Infect Dis. 1997 Dec;16(12):893-7. doi: 10.1007/BF01700555.
Four thousand eighty-eight Staphylococcus aureus isolates obtained from patients hospitalised in a university clinic and four community hospitals over a period of one year were screened for methicillin resistance. A resistance rate of 5% was detected among initial isolates. Distribution of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus showed an increased prevalence of MRSA in clinically significant specimens such as blood, central venous catheter tips, bronchial secretions, and wound secretions. Typing of 110 MRSA strains (initial isolates) by macrorestriction analysis of chromosomal DNA revealed 26 different genotypes that could be divided into five epidemic and 21 sporadic strains. More than 50% of all isolates belonged to one type that was confirmed to be closely related to the "southern-German" epidemic strain. Production of virulence factors such as enterotoxin A-D and toxic shock syndrome-toxin 1 among MRSA strains (initial isolates) occurred in ten of 26 different MRSA types. A strong correlation between genotype and toxin production was demonstrated.
在一年时间里，对从一所大学诊所和四家社区医院住院患者中分离出的4088株金黄色葡萄球菌进行了耐甲氧西林检测。初始分离株的耐药率为5%。耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感金黄色葡萄球菌的分布显示，在血液、中心静脉导管尖端、支气管分泌物和伤口分泌物等具有临床意义的标本中，MRSA的患病率有所增加。通过对染色体DNA进行宏观限制性分析，对110株MRSA菌株（初始分离株）进行分型，发现了26种不同的基因型，可分为5种流行株和21种散发株。所有分离株中超过50%属于一种类型，经证实与“德国南部”流行株密切相关。在26种不同的MRSA类型中，有10种MRSA菌株（初始分离株）产生了诸如肠毒素A - D和中毒性休克综合征毒素1等毒力因子。基因型与毒素产生之间存在很强的相关性。
