Durak I, Karabacak H I, Büyükkoçak S, Cimen M Y, Kaçmaz M, Omeroglu E, Oztürk H S
Biochemistry Department, Ankara University Medical Faculty, Turkey.
Nephron. 1998;78(2):207-11. doi: 10.1159/000044912.
Enzymatic antioxidant defense system and antioxidant defense potential (AOP) were studied in kidney tissue from rabbits treated with cyclosporine (CsA, 25 mg/kg/day), antioxidant vitamins (E, 100 mg/kg/day plus C, 200 mg/ kg/day), and CsA plus antioxidant vitamins, and in kidney tissue from control animals. Although no change was observed in superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) and catalase (CAT) activities were found decreased in kidney tissue exposed to CsA for 10 days compared with control tissue. The level of thiobarbituric acid-reagent substances (TBARS) was higher and antioxidant defense potential (AOP) lower in the CsA-treated group compared with the other groups. Histopathological examination reveals important subcellular damage in the renal tissue from the animals treated with CsA. Antioxidant vitamin therapy caused full improvement in the enzyme activities, TBARS levels and AOP, but the subcellular damage was partly ameliorated in the CsA plus vitamin group. Results suggest that CsA impairs the antioxidant defense system and reduces the antioxidant defense potential in the renal tissue. Antioxidant vitamin treatment protects the tissue in part against toxic effects of the drug.
在接受环孢素（CsA，25毫克/千克/天）、抗氧化维生素（E，100毫克/千克/天加C，200毫克/千克/天）以及CsA加抗氧化维生素治疗的兔子的肾脏组织中，以及在对照动物的肾脏组织中，研究了酶促抗氧化防御系统和抗氧化防御潜力（AOP）。虽然超氧化物歧化酶（SOD）活性未观察到变化，但与对照组织相比，暴露于CsA 10天的肾脏组织中的谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）活性降低。与其他组相比，CsA治疗组的硫代巴比妥酸反应物（TBARS）水平更高，抗氧化防御潜力（AOP）更低。组织病理学检查显示，接受CsA治疗的动物的肾组织存在重要的亚细胞损伤。抗氧化维生素疗法使酶活性、TBARS水平和AOP完全恢复正常，但在CsA加维生素组中，亚细胞损伤仅部分得到改善。结果表明，CsA损害了肾脏组织中的抗氧化防御系统并降低了抗氧化防御潜力。抗氧化维生素治疗部分保护组织免受药物的毒性作用。
