Joseph G T, McCarthy J S, Huima T, Mair K F, Kass P H, Boussinesq M, Goodrick L, Bradley J E, Lustigman S
Virology and Parasitology, The Lindsley F. Kimball Research Institute, New York Blood Center, NY 10021, USA.
Mol Biochem Parasitol. 1997 Dec 1;90(1):55-68. doi: 10.1016/s0166-6851(97)00132-1.
A pool of sera from individuals classified as putatively immune (PI) to Onchocerca volvulus infection was employed in the screening of a fourth-stage larval cDNA expression library. A highly immunogenic clone, encoding the Ov 53/80 protein, was identified. The full length cDNA of clone 4.21 contained 2527 nucleotides encoding 769 amino acids of which 100 are glutamine residues (13%). Antibodies raised against recombinant protein encoded by a partial cDNA sequence (clone 73-k) recognized a 53 and 80 kDa protein in O. volvulus larval and adult parasite extracts, respectively. The antibodies localized the native protein in the cuticle, hypodermis, secretory vesicles and in granules of the glandular esophagus of larvae and in the hypodermis and the cuticle of adult worms. The recombinant 73-k polypeptide (r73) was recognized by 90-100% of sera from PI and infected individuals from Liberia, but only by 67% of similar groups from Ecuador. r73 specific IgG2 and IgG3 levels in the PI from Liberia and Ecuador, respectively, were significantly lower than in the infected, whereas the r73 specific IgG1/IgG3 or IgG1/IgG2 in the PI and the infected individuals from Liberia or Ecuador, respectively, were similar. The IgG4 specific antibody response in the PI from Liberia and Ecuador were lower than in the infected. The T-cell proliferative responses to r73 in infected individuals from Cameroon were found to be inversely correlated with their levels of microfilariae.
从被分类为对盘尾丝虫感染具有推定免疫力（PI）的个体中获取的一组血清，用于筛选四期幼虫cDNA表达文库。鉴定出一个编码Ov 53/80蛋白的高免疫原性克隆。克隆4.21的全长cDNA包含2527个核苷酸，编码769个氨基酸，其中100个是谷氨酰胺残基（13%）。针对部分cDNA序列（克隆73-k）编码的重组蛋白产生的抗体，分别在盘尾丝虫幼虫和成虫寄生虫提取物中识别出53 kDa和80 kDa的蛋白。这些抗体将天然蛋白定位在幼虫的角质层、皮下组织、分泌小泡和腺食管颗粒中，以及成虫的皮下组织和角质层中。来自利比里亚的PI和感染个体的90%-100%的血清能识别重组73-k多肽（r73），但来自厄瓜多尔的类似群体中只有67%能识别。利比里亚和厄瓜多尔的PI中r73特异性IgG2和IgG3水平分别显著低于感染者，而利比里亚或厄瓜多尔的PI和感染个体中r73特异性IgG1/IgG3或IgG1/IgG2水平相似。利比里亚和厄瓜多尔的PI中IgG4特异性抗体反应低于感染者。在喀麦隆的感染个体中，发现对r73的T细胞增殖反应与其微丝蚴水平呈负相关。
