Lustigman S, Brotman B, Johnson E H, Smith A B, Huima T, Prince A M
Laboratory of Virology and Parasitology, Lindsley F. Kimball Research Institute of the New York Blood Center, NY 10021.
Mol Biochem Parasitol. 1992 Jan;50(1):79-93. doi: 10.1016/0166-6851(92)90246-g.
The identification and characterization of a recombinant cDNA clone (OV103) expressing a microfilarial surface-associated antigen of Onchocerca volvulus is described. OV103 was identified and isolated from a lambda gt11 cDNA expression library derived from adult O. volvulus mRNA using a chimpanzee antiserum, taken 2 years after infection with third-stage larvae of O. volvulus. The cDNA clone encodes a 12.5-kDa protein that corresponds to a 15-kDa parasite protein present in microfilariae and adult female worms. The antigen encoded by this clone is located in the basal layer of the cuticle and the hypodermis of the female adult worm, and on the surface of microfilariae. OV103 fusion polypeptide is recognized only by some sera from onchocerciasis infected subjects (57%), but more significantly (89%) by sera from individuals that have low levels of patent infection. In addition, the antibody response to this protein developed before appearance of microfilariae in the skin of chimpanzees that had developed non-patent or low level patent infections, while the antibody response in chimpanzees with high levels of microfilariae appeared later at the time of appearance of microfilariae. Preliminary experiments indicated that affinity purified antibodies directed against OV103 fusion polypeptide mediated killing of nodular microfilariae in vitro in the presence of normal peripheral blood granulocytes.
本文描述了一个表达盘尾丝虫微丝蚴表面相关抗原的重组cDNA克隆（OV103）的鉴定和特性。OV103是从一个λgt11 cDNA表达文库中鉴定并分离出来的，该文库源自盘尾丝虫成虫的mRNA，使用的是一只感染盘尾丝虫三期幼虫两年后的黑猩猩抗血清。该cDNA克隆编码一种12.5 kDa的蛋白质，它对应于微丝蚴和成年雌虫中存在的一种15 kDa的寄生虫蛋白。该克隆编码的抗原位于成年雌虫角质层和皮下组织的基底层以及微丝蚴的表面。OV103融合多肽仅被一些盘尾丝虫病感染患者的血清（57%）识别，但更显著的是被低度显性感染个体的血清（89%）识别。此外，在未出现显性感染或低度显性感染的黑猩猩皮肤中，在微丝蚴出现之前就对这种蛋白质产生了抗体反应，而在微丝蚴数量较多的黑猩猩中，抗体反应在微丝蚴出现时较晚出现。初步实验表明，针对OV103融合多肽的亲和纯化抗体在正常外周血粒细胞存在的情况下，可在体外介导结节性微丝蚴的杀伤。
