Beuzard Y, Vainchenker W, Testa U, Dubart A, Monplaisir N, Breton-Gorius J, Rosa J
Am J Hematol. 1979;7(3):207-18. doi: 10.1002/ajh.2830070304.
Erythroid burst colonies derived from the cord blood of nine neonates and from the blood and liver of three fetuses aborted after 20 weeks of gestation were grown in plasma clot culture. Their quantitative study revealed a higher proportion of burst-forming units (BFU-Es) in cord blood than in cord blood of normal adults. In addition, colony-forming units (CFU-Es) were present in cord blood but absent from adult blood. Study of haemoglobin synthesis in 14-day cultures of cord blood BFU-Es showed a significantly higher degree of Hb A synthesis than was found in reticulocytes from fresh cord blood; this proportion was, however, similar to that expected in vivo about three weeks after birth. These data suggest that the hemoglobin switch is already programmed in most of the early erythroid precursors present in cord blood or full-term neonates and indicate that the differentiation time is probably of the same order of magnitude in vivo and in vitro. The proportion of Hb A and F synthesis in erythroid bursts was not influenced by the concentration of erythropoietin in the range studied--ie, from 0.5 to 12 international units. Low but identical proportions of Hb A synthesis were found both in erythroid cells from liver after two hours of incubation with [3H]-leucine, and in 14-day liver bursts from fetuses aborted at 20 weeks of gestation.
从9名新生儿的脐带血以及3名妊娠20周后流产胎儿的血液和肝脏中获取红系爆式集落，在血浆凝块培养中进行培养。对它们的定量研究显示，脐带血中爆式形成单位（BFU-E）的比例高于正常成年人的脐带血。此外，脐带血中存在集落形成单位（CFU-E），而成年人血液中则没有。对脐带血BFU-E进行14天培养后的血红蛋白合成研究表明，其Hb A合成程度显著高于新鲜脐带血网织红细胞中的水平；然而，这一比例与出生后约三周体内预期的比例相似。这些数据表明，血红蛋白转换在脐带血或足月新生儿中存在的大多数早期红系前体细胞中已被编程，并表明体内和体外的分化时间可能处于相同的数量级。在所研究的促红细胞生成素浓度范围（即0.5至12国际单位）内红系爆式集落中Hb A和F合成的比例不受影响。在用[3H] - 亮氨酸孵育两小时后的肝脏红系细胞以及妊娠20周流产胎儿的14天肝脏爆式集落中，均发现了低但相同比例的Hb A合成。
