Yamada Y, Ohira H, Iwase H, Takatori T, Nagao M, Ohtani S
Department of Forensic Dental Medicine, Kanagawa Dental College, Yokosuka, Japan.
J Forensic Odontostomatol. 1997 Jun;15(1):13-6.
Genetic identification can be complicated by long intervals between the time of death and examination of tissues, and sometimes only bone and teeth may be available for analysis. Several investigators have described the isolation of nuclear DNA from these materials, but all have indicated that the DNA is significantly degraded. Recently, the polymerase chain reaction (PCR) and direct DNA sequencing have enabled rapid and reliable characterization of specific highly polymorphic DNA sequences from different individuals. Above all, mitochondrial DNA sequences offer several unique advantages for the identification of human remains. The isolation of mtDNA from a tooth and the symmetrical PCR amplification and direct DNA sequencing of its most polymorphic regions are reported.
死亡时间与组织检查之间间隔较长可能会使基因鉴定变得复杂，有时可能仅有骨骼和牙齿可供分析。几位研究人员描述了从这些材料中分离核DNA的方法，但均指出DNA已严重降解。最近，聚合酶链反应（PCR）和直接DNA测序已能够快速、可靠地鉴定不同个体中特定的高度多态性DNA序列。最重要的是，线粒体DNA序列在人类遗骸鉴定方面具有几个独特的优势。本文报道了从一颗牙齿中分离线粒体DNA及其最具多态性区域的对称PCR扩增和直接DNA测序。
