Holland M M, Fisher D L, Mitchell L G, Rodriquez W C, Canik J J, Merril C R, Weedn V W
Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, Washington, DC.
J Forensic Sci. 1993 May;38(3):542-53.
Deoxyribonucleic acid (DNA) sequence analysis of the control region of the mitochondrial DNA (mtDNA) genome was used to identify human skeletal remains returned to the United States government by the Vietnamese government in 1984. The postmortem interval was thought to be 24 years at the time of testing, and the remains presumed to be an American service member. DNA typing methods using nuclear genomic DNA, HLA-DQ alpha and the variable number of tandem repeat (VNTR) locus D1S80, were unsuccessful using the polymerase chain reaction (PCR). Amplification of a portion of the mtDNA control region was performed, and the resulting PCR product subjected to DNA sequence analysis. The DNA sequence generated from the skeletal remains was identical to the maternal reference sequence, as well as the sequence generated from two siblings (sisters). The sequence was unique when compared to more than 650 DNA sequences found both in the literature and provided by personal communications. The individual sequence polymorphisms were present in only 23 of the more than 1300 nucleotide positions analyzed. These results support the observation that in cases where conventional DNA typing is unavailable, mtDNA sequencing can be used for human remains identification.
线粒体DNA（mtDNA）基因组控制区的脱氧核糖核酸（DNA）序列分析被用于鉴定1984年越南政府归还给美国政府的人类骨骼遗骸。检测时推测死后间隔时间为24年，遗骸被认定为一名美国军人。使用聚合酶链反应（PCR）对核基因组DNA、HLA-DQα以及可变数目串联重复序列（VNTR）位点D1S80进行DNA分型的方法均未成功。对mtDNA控制区的一部分进行了扩增，并对得到的PCR产物进行DNA序列分析。从骨骼遗骸中生成的DNA序列与母系参考序列以及从两名姐妹生成的序列相同。与文献中以及个人交流提供的650多个DNA序列相比，该序列是独一无二的。在分析的1300多个核苷酸位置中，个体序列多态性仅出现在23个位置。这些结果支持了以下观点：在无法进行常规DNA分型的情况下，mtDNA测序可用于人类遗骸鉴定。
