Musmanno R A, Russi M, Figura N, Guglielmetti P, Zanchi A, Signori R, Rossolini A
Dipartimento di Biologia Molecolare, Università degli Studi, Siena, Italy.
New Microbiol. 1998 Jan;21(1):15-22.
From January 1989 to December 1990, stool samples from 288 children with enteritis were examined for the presence of unusual campylobacters which represented about 20% of all campylobacteria isolated when the filtration technique was used. The isolation percentage was the following: C. jejuni ss. jejuni 6.9%; C. coli 2%; C. jejuni ss. doylei, C. upsaliensis and C. concisus each 0.7%. The atypical Campylobacter isolates were examined for their virulence characteristics. Toxin profiles based on cytotonic, cytotoxic and cytolethal distending factors were determined after analysis responses in Vero, CHO and HeLa cells. Adhesivity and invasivity tests were performed on Intestine 407 cells. No strain was cytotoxic. C. jejuni ss. doylei and C. concisus induced an elongation of CHO cells (a cytotonic-like effect). C. upsaliensis strains provoked a cytolethal distending effect. No strain adhered to cells in vitro. Our results suggest that the filtration technique is excellent for the isolation of atypical campylobacters and indicate that the unusual Campylobacter isolates could be potentially virulent.
1989年1月至1990年12月，对288名肠炎患儿的粪便样本进行检查，以检测是否存在异常弯曲杆菌。当使用过滤技术时，这些异常弯曲杆菌约占所有分离出的弯曲杆菌的20%。分离百分比情况如下：空肠弯曲菌空肠亚种6.9%；结肠弯曲菌2%；空肠弯曲菌多氏亚种、乌普萨拉弯曲菌和简明弯曲菌各占0.7%。对非典型弯曲杆菌分离株的毒力特征进行了检测。在分析Vero、CHO和HeLa细胞的反应后，确定了基于细胞紧张性、细胞毒性和细胞致死性扩张因子的毒素谱。对肠407细胞进行了黏附性和侵袭性试验。没有菌株具有细胞毒性。空肠弯曲菌多氏亚种和简明弯曲菌可使CHO细胞伸长（类似细胞紧张性效应）。乌普萨拉弯曲菌菌株可引起细胞致死性扩张效应。没有菌株在体外能黏附细胞。我们的结果表明，过滤技术对于分离非典型弯曲杆菌非常有效，并且表明这些异常弯曲杆菌分离株可能具有潜在毒性。
