Cavalla P, Dutto A, Piva R, Richiardi P, Grosso R, Schiffer D
Department of Neuroscience, University of Turin, Italy.
Acta Neuropathol. 1998 Feb;95(2):131-5. doi: 10.1007/s004010050776.
Cyclin D1 (cycD1) expression was defined immunohistochemically using monoclonal antibody DCS-6 and polyclonal antiserum H-295 in 50 glioma biopsies. The number of positive nuclei was higher for H-295 than for DCS-6, with a ratio of 3:1. The labelling index (LI) was compared to the grade of histological malignancy and to Ki-67 MIB-1 LI. The LI for cycD1 increased with histological malignancy, in parallel with the increase in MIB-1 LI. In most tumours, the maximum LI for cycD1 and MIB-1 were found in the same areas. The mean MIB-1 LI: mean cycD1 LI ratio does not vary in the three grades of astrocytic tumours. However, in this study the correlation between the two LIs was not statistically significant. Staining for cycD1 antigen does not necessarily imply that the gene is overexpressed since other molecular mechanisms can also be responsible for cell cycle deregulation. In invasive areas, the cycD1 LI is frequently higher than in solid tumour, either because more tumour cells are positive or because reactive astrocytes and activated microglia express cycD1. The relative contribution of neoplastic and reactive cells remains to be defined.
使用单克隆抗体DCS - 6和多克隆抗血清H - 295对50例胶质瘤活检组织进行免疫组织化学检测，以确定细胞周期蛋白D1（cycD1）的表达情况。H - 295阳性细胞核数量高于DCS - 6，比例为3:1。将标记指数（LI）与组织学恶性程度以及Ki - 67 MIB - 1 LI进行比较。cycD1的LI随组织学恶性程度增加而升高，与MIB - 1 LI的增加平行。在大多数肿瘤中，cycD1和MIB - 1的最大LI出现在相同区域。星形细胞瘤的三个级别中，平均MIB - 1 LI与平均cycD1 LI的比值无变化。然而，在本研究中，这两个LI之间的相关性无统计学意义。cycD1抗原染色并不一定意味着该基因过度表达，因为其他分子机制也可能导致细胞周期失调。在浸润区域，cycD1 LI通常高于实体瘤，这可能是因为更多肿瘤细胞呈阳性，或者是因为反应性星形胶质细胞和活化的小胶质细胞表达cycD1。肿瘤细胞和反应性细胞的相对贡献仍有待确定。
