Chakrabarty A, Bridges L R, Gray S
Neuropathology Laboratory, General Infirmary at Leeds, University of Leeds, UK.
Neuropathol Appl Neurobiol. 1996 Aug;22(4):311-6. doi: 10.1111/j.1365-2990.1996.tb01109.x.
Forty-eight astrocytic tumours were stained immunohistochemically with antibodies to the cell cycle-regulating protein, cyclin D1, and to the proliferation marker MIB1 (Ki-67) using formalin fixed paraffin embedded tissue and a microwave antigen retrieval system. Cases were classified by the WHO system (1993). The labelling indices (LI) for both antibodies were compared with each other and with the tumour type. The mean labelling indices for both antibodies increased with the degree of malignancy, and a significant difference was seen between the pilocytic astrocytoma and diffuse astrocytoma together vs anaplastic astrocytoma and glioblastoma together. However, within each tumour type there was considerable variation in the labelling indices and a clear cut off value could not be demonstrated. There was a strong positive correlation between labelling indices for cyclin D1 and MIB1 in diffuse astrocytoma, but this correlation broke down increasingly in anaplastic astrocytoma and glioblastoma. There was poor correlation between cyclin D1 and MIB1 in pilocytic astrocytoma, a feature which appeared to separate them from the diffuse astrocytoma. Average labelling indices for cyclin D1 were higher than those of MIB1, which suggests that cyclin D1 positive cells represent a pool of cells from which proliferation and hence MIB1 expression can take place. In conclusion, cyclin D1 is overexpressed in astrocytic tumours, more so with increasing grade of malignancy and in a way which approximately correlates with MIB1 expression.
采用福尔马林固定石蜡包埋组织和微波抗原修复系统，用细胞周期调节蛋白细胞周期蛋白D1抗体和增殖标志物MIB1（Ki-67）抗体对48例星形细胞瘤进行免疫组织化学染色。病例根据世界卫生组织系统（1993年）进行分类。将两种抗体的标记指数（LI）相互比较，并与肿瘤类型进行比较。两种抗体的平均标记指数均随恶性程度增加而升高，在毛细胞型星形细胞瘤和弥漫性星形细胞瘤与间变性星形细胞瘤和胶质母细胞瘤之间存在显著差异。然而，在每种肿瘤类型中，标记指数存在相当大的差异，且无法证明有明确的临界值。弥漫性星形细胞瘤中细胞周期蛋白D1和MIB1的标记指数之间存在强正相关，但在间变性星形细胞瘤和胶质母细胞瘤中这种相关性逐渐减弱。毛细胞型星形细胞瘤中细胞周期蛋白D1和MIB1之间的相关性较差，这一特征似乎将它们与弥漫性星形细胞瘤区分开来。细胞周期蛋白D1的平均标记指数高于MIB1，这表明细胞周期蛋白D1阳性细胞代表了一组可发生增殖并因此表达MIB1的细胞。总之，细胞周期蛋白D1在星形细胞瘤中过度表达，在恶性程度增加时更为明显，且在某种程度上与MIB1表达大致相关。
