Schosinsky K H, Chavarria M, Lehmann H P
Clin Chem. 1976 Aug;22(8):1339-42.
A micromethod for the analysis for chloride, based on the chemical precipitation of silver chloride by radial diffusion through agar gel containing silver nitrate, is described. The method is simple to run, requires little or no instrumentation, and requires only 10 mul of sample. Results by coulometric titration (Buchler Cotlove Chloridometer) correlated well for serum (r = 0.961), urine (r = 0.997), cerebrospinal fluid (r = 0.991), and sweat (r = 0.998). Other halide ions or protein do not interfere. Precision studies gave a within-day reproducibility (CV) of 1.3% and a day-to-day variability of 2.1% for a serum sample averaging 115 mmol/liter.
本文描述了一种基于氯化银通过含有硝酸银的琼脂凝胶径向扩散进行化学沉淀来分析氯化物的微量方法。该方法操作简单，几乎不需要仪器设备，且仅需10微升样品。通过库仑滴定法（布赫勒·科特洛夫氯化物测定仪）得到的结果与血清（r = 0.961）、尿液（r = 0.997）、脑脊液（r = 0.991）和汗液（r = 0.998）的结果相关性良好。其他卤离子或蛋白质不产生干扰。对平均浓度为115毫摩尔/升的血清样品进行的精密度研究得出，日内重复性（CV）为1.3%，日间变异性为2.1%。
