Ressler N, Gashkoff M, Fischinger A
Clin Chem. 1976 Aug;22(8):1355-60.
We examined the feasibility of measuring absorbance in the far ultraviolet for serum protein determination with a medium-priced, double-beam spectrophotometer. The method has been simplified by using an automatic dilutor to dilute the serum, and a flow-through cell and recorder in conjunction with the spectrophotometer. Because it is only necessary to dilute the serum and determine its absorbance, the procedure is quite rapid. As compared to the biuret reaction, interferences from hemolysis have been about halved. Interferences owing to light scattering by turbid sera have also been appreciably decreased by use of an anionic detegent (sodium dodecyl sulfate) as a serum diluent. Comparisons of the present method to the biuret and Kjeldahl methods indicate that it is relatively accurate, and is practical for routine use.
我们用一台中等价位的双光束分光光度计研究了在远紫外区测量吸光度以测定血清蛋白的可行性。该方法通过使用自动稀释器稀释血清，并结合分光光度计使用流通池和记录仪得以简化。由于仅需稀释血清并测定其吸光度，所以该程序相当快速。与双缩脲反应相比，溶血干扰大约减半。通过使用阴离子去污剂（十二烷基硫酸钠）作为血清稀释剂，浑浊血清引起的光散射干扰也显著降低。将本方法与双缩脲法和凯氏定氮法进行比较表明，它相对准确，适用于常规使用。
