Gründer W, Wagner M, Werner A
Universität Leipzig, Medizinische Fakultät, Institut für Medizinische Physik und Biophysik, Germany.
Magn Reson Med. 1998 Mar;39(3):376-82. doi: 10.1002/mrm.1910390307.
NMR microscopic studies of articular cartilage at 7.1 T are presented. Using a special experimental design, T2-weighted spin-echo images of cartilage-bone plugs were taken under variable angles with respect to the static magnetic field B0 to visualize the angular-dependent representation of internal matrix structures mediated by the collagen network arrangement. To quantify the observed orientational effect in the MR images, exact measurements of the transverse relaxation time T2 were taken using the CPMG sequence. The NMR experiments show the strong influence of the cartilage orientation with respect to the static magnetic field on the inhomogeneous appearance of the articular cartilage in the MR image. Additionally performed polarization light microscopic investigations demonstrate the direct relation between the oriented collagenous structures and the anisotropic regions observed in the MR images. A simple cartilage matrix model derived from the experimental findings is proposed, and consequences for the clinical assessment of the articular joint are discussed.
本文展示了在7.1T磁场下对关节软骨的核磁共振显微镜研究。采用特殊的实验设计，以相对于静磁场B0的可变角度拍摄软骨-骨栓的T2加权自旋回波图像，以可视化由胶原网络排列介导的内部基质结构的角度依赖性表现。为了量化磁共振图像中观察到的取向效应，使用CPMG序列对横向弛豫时间T2进行了精确测量。核磁共振实验表明，软骨相对于静磁场的取向对磁共振图像中关节软骨的不均匀外观有很大影响。另外进行的偏振光显微镜研究证明了定向胶原结构与磁共振图像中观察到的各向异性区域之间的直接关系。根据实验结果提出了一个简单的软骨基质模型，并讨论了其对关节临床评估的影响。
