Sudo Y, Miyagawa R, Takahata Y
Chemicals Inspection and Testing Institute, Division of Research and Development, Tokyo, Japan.
J Chromatogr B Biomed Sci Appl. 1998 Jan 23;705(1):55-62. doi: 10.1016/s0378-4347(97)00496-9.
A new method for the preparation of restricted access media (RAM) was developed. A low-temperature plasma treatment removed octadecyl groups on the external surface of octadecylsilylated silica gel before those on the internal surface to produce silanol groups. The silanol groups produced were glycerylpropylsilylated to give RAM, DIOL-ODSs, consisting of internal octadecyl groups and external glycerylpropyl groups. DIOL-ODSs were inert to serum proteins and retained low-molecular-mass analytes adequately. Direct injection analysis of anticonvulsants in serum was accurately performed by column-switching high-performance liquid chromatography using a pre-column packed with DIOL-ODS.
开发了一种制备受限进样介质（RAM）的新方法。低温等离子体处理在十八烷基硅烷化硅胶内表面的十八烷基被去除之前，先去除其外表面的十八烷基，以产生硅醇基。将产生的硅醇基进行甘油丙基硅烷化，得到由内部十八烷基和外部甘油丙基组成的RAM，即二醇基十八烷基硅胶（DIOL-ODSs）。DIOL-ODSs对血清蛋白呈惰性，并能充分保留低分子量分析物。使用填充有DIOL-ODS的预柱，通过柱切换高效液相色谱法准确地对血清中的抗惊厥药进行直接进样分析。
