Verhagen C E, van der Pouw Kraan T C, Buffing A A, Chand M A, Faber W R, Aarden L A, Das P K
Department of Dermatology, Academic Medical Center, University of Amsterdam, The Netherlands.
J Immunol. 1998 Mar 1;160(5):2380-7.
In an earlier study, we generated a large number of Mycobacterium leprae-responsive and M. leprae-nonresponsive T cell clones (TCC) from the lesional skin of immunologic unstable borderline leprosy patients. In that study, we divided TCC into type 1- and type 2-like on the basis of their IFN-gamma and IL-4 expression. To explore whether other cytokines are coproduced along with IFN-gamma and IL-4, we investigated the secretion of a panel of other cytokines (TNF-alpha, IL-5, IL-6, IL-10, and IL-13) by a large number of these TCC. Upon analysis of 139 M. leprae-responsive TCC, we observed a positive correlation in the coproduction of IFN-gamma/TNF-alpha (r = 0.81), and in that of IL-4/IL-5 (r = 0.83), IL-4/IL-13 (r = 0.80), and IL-5/IL-13 (r = 0.82). Polarized type 1-like TCC produced dominantly IFN-gamma/TNF-alpha, and polarized type 2-like TCC predominantly IL-4/IL-5/IL-13. Most type 0-like TCC produced both sets of cytokines. In contrast, type 1- and type 2-like subsets of M. leprae-nonresponsive TCC (n = 58) did not show the same coexpression of these cytokines. Furthermore, when the differential expression of a broad panel of cytokines by individual M. leprae-responsive TCC is considered, it appeared that additional phenotypes could be recognized. These results suggested that distinct isotypes of type 1- and type 2-like T cells, based on the secretion of a panel of cytokines, may reflect M. leprae-specific characteristics.
在一项早期研究中，我们从免疫不稳定的界线类麻风患者的皮损中生成了大量对麻风分枝杆菌有反应和无反应的T细胞克隆（TCC）。在该研究中，我们根据TCC的γ干扰素和白细胞介素-4表达将其分为1型和2型样TCC。为了探究是否有其他细胞因子与γ干扰素和白细胞介素-4共同产生，我们研究了大量这些TCC对一组其他细胞因子（肿瘤坏死因子-α、白细胞介素-5、白细胞介素-6、白细胞介素-10和白细胞介素-13）的分泌情况。在分析139个对麻风分枝杆菌有反应的TCC时，我们观察到γ干扰素/肿瘤坏死因子-α的共同产生呈正相关（r = 0.81），白细胞介素-4/白细胞介素-5（r = 0.83）、白细胞介素-4/白细胞介素-13（r = 0.80）以及白细胞介素-5/白细胞介素-13（r = 0.82）的共同产生也呈正相关。极化的1型样TCC主要产生γ干扰素/肿瘤坏死因子-α，极化的2型样TCC主要产生白细胞介素-4/白细胞介素-5/白细胞介素-13。大多数0型样TCC产生这两组细胞因子。相比之下，对麻风分枝杆菌无反应的TCC（n = 58）的1型和2型样亚群并未表现出这些细胞因子的相同共表达情况。此外，当考虑单个对麻风分枝杆菌有反应的TCC对一大组细胞因子的差异表达时，似乎可以识别出其他表型。这些结果表明，基于一组细胞因子的分泌，1型和2型样T细胞的不同亚型可能反映麻风分枝杆菌特异性特征。
