托戈托正黏液病毒病毒粒子RNA和互补RNA启动子中的一种核酸内切酶切换机制。
An endonuclease switching mechanism in the virion RNA and cRNA promoters of Thogoto orthomyxovirus.
作者信息
Leahy M B, Dessens J T, Pritlove D C, Nuttall P A
机构信息
NERC Institute of Virology and Environmental Microbiology, Oxford, United Kingdom.
出版信息
J Virol. 1998 Mar;72(3):2305-9. doi: 10.1128/JVI.72.3.2305-2309.1998.
Abstract
An in vitro assay was developed to investigate endonuclease activity of Thogoto virus, a tick-borne orthomyxovirus. Endonuclease activity relied on an interaction between the 3' and 5' termini of virion RNA (vRNA) and not those of cRNA. Evidence was obtained that cap structures are cleaved directly from cap donors and that cleavage does not occur after pyrimidines. A 5' hook structure, present in the vRNA promoter but not the cRNA promoter, was introduced into cRNA promoter mutants. These mutants stimulated endonuclease activity, although at levels slightly lower than that of vRNA. The ability of the cRNA promoter to stimulate endonuclease activity when mutated to contain a 5' hook structure indicates that this structure constitutes a switching mechanism for endonuclease activity between the vRNA and cRNA promoters.
摘要
开发了一种体外测定法来研究托戈托病毒（一种蜱传正粘病毒）的核酸内切酶活性。核酸内切酶活性依赖于病毒体RNA（vRNA）的3'和5'末端之间的相互作用，而不是cRNA的3'和5'末端之间的相互作用。有证据表明帽结构是直接从帽供体上切割下来的，并且切割不会在嘧啶之后发生。将存在于vRNA启动子而非cRNA启动子中的5'钩状结构引入cRNA启动子突变体中。这些突变体刺激了核酸内切酶活性，尽管其水平略低于vRNA。当cRNA启动子突变以包含5'钩状结构时刺激核酸内切酶活性的能力表明该结构构成了vRNA和cRNA启动子之间核酸内切酶活性的转换机制。
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本文引用的文献
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2
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3
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