应用编码Fas配体的重组腺病毒载体延长转基因表达。
Application of a Fas ligand encoding a recombinant adenovirus vector for prolongation of transgene expression.
作者信息
Zhang H G, Bilbao G, Zhou T, Contreras J L, Gómez-Navarro J, Feng M, Saito I, Mountz J D, Curiel D T
机构信息
Gene Therapy Program, Department of Rheumatology, University of Alabama at Birmingham, 35294, USA.
出版信息
J Virol. 1998 Mar;72(3):2483-90. doi: 10.1128/JVI.72.3.2483-2490.1998.
Abstract
An adenovirus vector encoding murine Fas ligand (mFasL) under an inducible control was derived. In vivo ectopic expression of mFasL in murine livers induced an inflammatory cellular infiltration. Furthermore, ectopic expression of mFasL by myocytes did not allow prolonged vector-mediated transgene expression. Thus, ectopic expression of functional mFasL in vector-transduced cells does not appear to confer, by itself, an immunoprivileged site sufficient to mitigate adenovirus vector immunogenicity.
摘要
构建了一种在诱导性控制下编码小鼠Fas配体（mFasL）的腺病毒载体。mFasL在小鼠肝脏中的体内异位表达诱导了炎性细胞浸润。此外，肌细胞中mFasL的异位表达不允许载体介导的转基因进行长期表达。因此，在载体转导的细胞中功能性mFasL的异位表达本身似乎并不能赋予一个足以减轻腺病毒载体免疫原性的免疫赦免位点。
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