• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

应用编码Fas配体的重组腺病毒载体延长转基因表达。

Application of a Fas ligand encoding a recombinant adenovirus vector for prolongation of transgene expression.

作者信息

Zhang H G, Bilbao G, Zhou T, Contreras J L, Gómez-Navarro J, Feng M, Saito I, Mountz J D, Curiel D T

机构信息

Gene Therapy Program, Department of Rheumatology, University of Alabama at Birmingham, 35294, USA.

出版信息

J Virol. 1998 Mar;72(3):2483-90. doi: 10.1128/JVI.72.3.2483-2490.1998.

DOI:10.1128/JVI.72.3.2483-2490.1998
PMID:9499110
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC109549/
Abstract

An adenovirus vector encoding murine Fas ligand (mFasL) under an inducible control was derived. In vivo ectopic expression of mFasL in murine livers induced an inflammatory cellular infiltration. Furthermore, ectopic expression of mFasL by myocytes did not allow prolonged vector-mediated transgene expression. Thus, ectopic expression of functional mFasL in vector-transduced cells does not appear to confer, by itself, an immunoprivileged site sufficient to mitigate adenovirus vector immunogenicity.

摘要

构建了一种在诱导性控制下编码小鼠Fas配体（mFasL）的腺病毒载体。mFasL在小鼠肝脏中的体内异位表达诱导了炎性细胞浸润。此外，肌细胞中mFasL的异位表达不允许载体介导的转基因进行长期表达。因此，在载体转导的细胞中功能性mFasL的异位表达本身似乎并不能赋予一个足以减轻腺病毒载体免疫原性的免疫赦免位点。

相似文献

1
Application of a Fas ligand encoding a recombinant adenovirus vector for prolongation of transgene expression.应用编码Fas配体的重组腺病毒载体延长转基因表达。
J Virol. 1998 Mar;72(3):2483-90. doi: 10.1128/JVI.72.3.2483-2490.1998.
2
Induction of specific T-cell tolerance by adenovirus-transfected, Fas ligand-producing antigen presenting cells.通过腺病毒转染的、产生Fas配体的抗原呈递细胞诱导特异性T细胞耐受。
Nat Biotechnol. 1998 Nov;16(11):1045-9. doi: 10.1038/3488.
3
Fas-Fas ligand interactions play a major role in effector functions of cytotoxic T lymphocytes after adenovirus vector-mediated gene transfer.在腺病毒载体介导的基因转移后，Fas-Fas配体相互作用在细胞毒性T淋巴细胞的效应功能中起主要作用。
Hum Gene Ther. 1999 Jan 20;10(2):259-69. doi: 10.1089/10430349950019048.
4
Adenoviral vector-mediated gene therapy in the mouse lung: no role of Fas-Fas ligand interactions for elimination of transgene expression in bronchioepithelial cells.
Hum Gene Ther. 1999 Nov 20;10(17):2839-46. doi: 10.1089/10430349950016564.
5
Long-lasting adenovirus transgene expression in mice through neonatal intrathymic tolerance induction without the use of immunosuppression.通过新生小鼠胸腺内诱导耐受实现腺病毒转基因在小鼠体内的长期表达，且无需使用免疫抑制剂。
J Virol. 1997 Jul;71(7):5330-5. doi: 10.1128/JVI.71.7.5330-5335.1997.
6
Construction of a recombinant adenovirus vector encoding Fas ligand with a CRE/Loxp inducible system.
Transplant Proc. 1999 Feb-Mar;31(1-2):792-3. doi: 10.1016/s0041-1345(98)01771-0.
7
On/off switching Fas-ligand gene expression in liver by Cre/Loxp adenovirus vector system.
Transplant Proc. 1999 Feb-Mar;31(1-2):753-4. doi: 10.1016/s0041-1345(98)01756-4.
8
Fulminant hepatitis by Fas-ligand expression in MRL-lpr/lpr mice grafted with Fas-positive livers and wild-type mice with Fas-mutant livers.在移植了Fas阳性肝脏的MRL-lpr/lpr小鼠和具有Fas突变肝脏的野生型小鼠中，通过Fas配体表达引发的暴发性肝炎。
Transplantation. 2001 Feb 27;71(4):503-8. doi: 10.1097/00007890-200102270-00004.
9
Fas-mediated apoptosis is involved in the elimination of gene-transduced hepatocytes with E1/E3-deleted adenoviral vectors.Fas介导的细胞凋亡参与了利用缺失E1/E3的腺病毒载体对基因转导肝细胞的清除过程。
J Gastroenterol Hepatol. 1998 Sep;13 Suppl:S113-8.
10
MRL/lpr CD4- CD8- and CD8+ T cells, respectively, mediate Fas-dependent and perforin cytotoxic pathways.MRL/lpr CD4 - CD8 - 和CD8 + T细胞分别介导Fas依赖性和穿孔素细胞毒性途径。
Eur J Immunol. 1997 Feb;27(2):415-20. doi: 10.1002/eji.1830270211.

引用本文的文献

1
Inflammation, apoptosis, and necrosis induced by neoadjuvant fas ligand gene therapy improves survival of dogs with spontaneous bone cancer.新辅助 Fas 配体基因治疗诱导的炎症、细胞凋亡和坏死可提高自发性骨肉瘤犬的生存率。
Mol Ther. 2012 Dec;20(12):2234-43. doi: 10.1038/mt.2012.149. Epub 2012 Jul 31.
2
Conditional gene expression systems to study herpesvirus biology in vivo.用于在体内研究疱疹病毒生物学的条件性基因表达系统。
Med Microbiol Immunol. 2008 Jun;197(2):269-76. doi: 10.1007/s00430-008-0086-1. Epub 2008 Mar 7.
3
Specific deletion of autoreactive T cells by adenovirus-transfected, Fas ligand-producing antigen-presenting cells.通过腺病毒转染的、产生Fas配体的抗原呈递细胞特异性清除自身反应性T细胞。
Immunol Res. 2002;26(1-3):235-46. doi: 10.1385/ir:26:1-3:235.
4
Hepatic DR5 induces apoptosis and limits adenovirus gene therapy product expression in the liver.肝脏中的死亡受体5（DR5）可诱导细胞凋亡并限制腺病毒基因治疗产物在肝脏中的表达。
J Virol. 2002 Jun;76(11):5692-700. doi: 10.1128/jvi.76.11.5692-5700.2002.
5
Efficient gene activation in cultured mammalian cells mediated by FLP recombinase-expressing recombinant adenovirus.表达FLP重组酶的重组腺病毒介导的培养哺乳动物细胞中的高效基因激活。
Nucleic Acids Res. 2001 Apr 1;29(7):E40. doi: 10.1093/nar/29.7.e40.
6
Antigen presenting cells expressing Fas ligand down-modulate chronic inflammatory disease in Fas ligand-deficient mice.表达Fas配体的抗原呈递细胞可下调Fas配体缺陷小鼠的慢性炎症性疾病。
J Clin Invest. 2000 Mar;105(6):813-21. doi: 10.1172/JCI8236.
7
Adenovirus-mediated gene expression in vivo is enhanced by the antiapoptotic bcl-2 gene.抗凋亡的bcl-2基因可增强腺病毒介导的体内基因表达。
J Virol. 1999 Aug;73(8):6992-7000. doi: 10.1128/JVI.73.8.6992-7000.1999.

本文引用的文献

1
Testis-derived Sertoli cells survive and provide localized immunoprotection for xenografts in rat brain.睾丸来源的支持细胞在大鼠脑内存活并为异种移植提供局部免疫保护。
Nat Biotechnol. 1996 Dec;14(13):1692-5. doi: 10.1038/nbt1296-1692.
2
Fas ligand expression in islets of Langerhans does not confer immune privilege and instead targets them for rapid destruction.胰岛中Fas配体的表达不会赋予免疫特权，反而会将胰岛作为快速破坏的靶点。
Nat Med. 1997 Jul;3(7):738-43. doi: 10.1038/nm0797-738.
3
Evasion of cytotoxic T lymphocyte (CTL) responses by nef-dependent induction of Fas ligand (CD95L) expression on simian immunodeficiency virus-infected cells.通过在感染猿猴免疫缺陷病毒的细胞上依赖nef诱导Fas配体（CD95L）表达来逃避细胞毒性T淋巴细胞（CTL）反应。
J Exp Med. 1997 Jul 7;186(1):7-16. doi: 10.1084/jem.186.1.7.
4
Adenovirus-mediated expression of Fas ligand induces hepatic apoptosis after Systemic administration and apoptosis of ex vivo-infected pancreatic islet allografts and isografts.
Hum Gene Ther. 1997 May 20;8(8):955-63. doi: 10.1089/hum.1997.8.8-955.
5
Possible participation of Fas-mediated apoptosis in the mechanism of atherosclerosis.Fas介导的细胞凋亡可能参与动脉粥样硬化的发病机制。
Gerontology. 1997;43 Suppl 1:35-42. doi: 10.1159/000213884.
6
Fas-ligand: privilege and peril.Fas配体：特权与风险。
Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):5986-90. doi: 10.1073/pnas.94.12.5986.
7
Role of Fas ligand (CD95L) in immune escape: the tumor cell strikes back.Fas配体（CD95L）在免疫逃逸中的作用：肿瘤细胞反击。
J Immunol. 1997 May 15;158(10):4521-4.
8
Transgenic expression of CD95 ligand on islet beta cells induces a granulocytic infiltration but does not confer immune privilege upon islet allografts.胰岛β细胞上CD95配体的转基因表达可诱导粒细胞浸润，但不能赋予胰岛同种异体移植物免疫特权。
Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3943-7. doi: 10.1073/pnas.94.8.3943.
9
Polymorphism of murine Fas ligand that affects the biological activity.影响生物活性的小鼠Fas配体多态性。
Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3914-9. doi: 10.1073/pnas.94.8.3914.
10
Thyroiditis and hepatitis: Fas on the road to disease.甲状腺炎与肝炎：Fas 在疾病发展过程中的作用
Nat Med. 1997 Apr;3(4):387-8. doi: 10.1038/nm0497-387.