人类免疫缺陷病毒（HIV）包膜可独立于CD4与CXCR4结合，与可溶性CD4相互作用或HIV包膜去糖基化可增强这种结合。
Human immunodeficiency virus (HIV) envelope binds to CXCR4 independently of CD4, and binding can be enhanced by interaction with soluble CD4 or by HIV envelope deglycosylation.
作者信息
Bandres J C, Wang Q F, O'Leary J, Baleaux F, Amara A, Hoxie J A, Zolla-Pazner S, Gorny M K
机构信息
Research Center for AIDS and HIV Infection, Manhattan VA Medical Center, and Department of Pathology, New York University, New York 10010, USA.
出版信息
J Virol. 1998 Mar;72(3):2500-4. doi: 10.1128/JVI.72.3.2500-2504.1998.
Abstract
Chemokine receptor CXCR4 (also known as LESTR and fusin) has been shown to function as a coreceptor for T-cell-tropic strains of human immunodeficiency virus type 1 (HIV-1). We have developed a binding assay to show that HIV envelope (Env) can interact with CXCR4 independently of CD4 but that this binding is markedly enhanced by the previous interaction of Env with soluble CD4. We also show that nonglycosylated HIV-1(SF-2) gp120 or sodium metaperiodate-treated oligomeric gp160 from HIV-1(451) bound much more readily to CXCR4 than their counterparts with intact carbohydrate residues did.
摘要
趋化因子受体CXCR4（也称为LESTR和融合素）已被证明可作为1型人类免疫缺陷病毒（HIV-1）嗜T细胞株的共受体。我们开发了一种结合试验，以表明HIV包膜（Env）可独立于CD4与CXCR4相互作用，但Env与可溶性CD4的先前相互作用可显著增强这种结合。我们还表明，非糖基化的HIV-1（SF-2）gp120或经高碘酸钠处理的来自HIV-1（451）的寡聚gp160比具有完整碳水化合物残基的对应物更容易与CXCR4结合。
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本文引用的文献
1
HIV coreceptor downregulation as antiviral principle: SDF-1alpha-dependent internalization of the chemokine receptor CXCR4 contributes to inhibition of HIV replication.作为抗病毒原理的HIV共受体下调：趋化因子受体CXCR4的SDF-1α依赖性内化有助于抑制HIV复制。
J Exp Med. 1997 Jul 7;186(1):139-46. doi: 10.1084/jem.186.1.139.
2
CD4-independent association between HIV-1 gp120 and CXCR4: functional chemokine receptors are expressed in human neurons.HIV-1糖蛋白120与CXCR4之间不依赖CD4的关联：功能性趋化因子受体在人类神经元中表达。
Curr Biol. 1997 Feb 1;7(2):112-21. doi: 10.1016/s0960-9822(06)00055-8.
3
CD4-independent infection by HIV-2 is mediated by fusin/CXCR4.HIV-2的非CD4依赖性感染由趋化因子/ CXCR4介导。
Cell. 1996 Nov 15;87(4):745-56. doi: 10.1016/s0092-8674(00)81393-8.
4
CD4-dependent, antibody-sensitive interactions between HIV-1 and its co-receptor CCR-5.HIV-1与其共受体CCR-5之间依赖CD4且对抗体敏感的相互作用。
Nature. 1996 Nov 14;384(6605):184-7. doi: 10.1038/384184a0.
5
CD4-induced interaction of primary HIV-1 gp120 glycoproteins with the chemokine receptor CCR-5.CD4诱导的原发性HIV-1 gp120糖蛋白与趋化因子受体CCR-5的相互作用。
Nature. 1996 Nov 14;384(6605):179-83. doi: 10.1038/384179a0.
6
Evidence for cell-surface association between fusin and the CD4-gp120 complex in human cell lines.人类细胞系中融合素与CD4-gp120复合物之间细胞表面关联的证据。
Science. 1996 Oct 25;274(5287):602-5. doi: 10.1126/science.274.5287.602.
7
The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1.CXC趋化因子SDF-1是LESTR/fusin的配体，可预防经T细胞系适应的HIV-1感染。
Nature. 1996 Aug 29;382(6594):833-5. doi: 10.1038/382833a0.
8
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Nature. 1996 Aug 29;382(6594):829-33. doi: 10.1038/382829a0.
9
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10
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