SCL/Tal-1转录因子在cloche基因的下游发挥作用，以确定斑马鱼中的造血和血管祖细胞。
SCL/Tal-1 transcription factor acts downstream of cloche to specify hematopoietic and vascular progenitors in zebrafish.
作者信息
Liao E C, Paw B H, Oates A C, Pratt S J, Postlethwait J H, Zon L I
机构信息
Division of Hematology/Oncology, Children's Hospital, Department of Pediatrics and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
出版信息
Genes Dev. 1998 Mar 1;12(5):621-6. doi: 10.1101/gad.12.5.621.
Abstract
SCL/Tal-1 is a transcription factor necessary for hematopoietic stem cell differentiation. Although SCL is also expressed in endothelial and neural progenitors, SCL function in these cells remains unknown. In the zebrafish mutant cloche (clo), SCL expression is nearly abolished in hematopoietic and vascular tissues. Correspondingly, it was shown previously that clo fails to differentiate blood and angioblasts. Genetic analysis demonstrates that the clo mutation is not linked to the SCL locus. Forced expression of SCL in clo embryos rescues the blood and vascular defects, suggesting that SCL acts downstream of clo to specify hematopoietic and vascular differentiation.
摘要
SCL/Tal-1是造血干细胞分化所必需的一种转录因子。尽管SCL也在内皮祖细胞和神经祖细胞中表达，但其在这些细胞中的功能仍不清楚。在斑马鱼突变体cloche（clo）中，造血组织和血管组织中的SCL表达几乎完全缺失。相应地，先前的研究表明，clo无法分化出血细胞和成血管细胞。遗传分析表明，clo突变与SCL基因座没有连锁关系。在clo胚胎中强制表达SCL可挽救血液和血管缺陷，这表明SCL在clo下游发挥作用，决定造血和血管分化。
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