Wiegand G, Kaleja R
Eur J Biochem. 1976 Jun 1;65(2):473-9. doi: 10.1111/j.1432-1033.1976.tb10363.x.
The synthesis and properties of strongly fluorescent derivative of inosine and its nucleotides are described. Reaction of 2-chloro-inosinic acid with sodium azide leads to a product bearing the tetrazole ring between position 2 and 3. Methylation at N1 was effected with dimethyl sulfate. The corresponding nucleosides and their 5'-triphosphates were also prepared. Only the non-methylated series is at equilibrium with small concentrations of their azido forms, and can be photolyzed by wavelengths above 300 nm. Both series are strongly fluorescent, their emission and excitation fluorescence spectra, as well as their quantum yields were measured. The nucleoside 5'-triphosphates are able to initiate and sustain polymerization of porcine brain tubulin.
描述了肌苷及其核苷酸的强荧光衍生物的合成与性质。2-氯肌苷酸与叠氮化钠反应生成在2位和3位之间带有四唑环的产物。用硫酸二甲酯在N1位进行甲基化。还制备了相应的核苷及其5'-三磷酸酯。只有未甲基化的系列与其叠氮形式的低浓度处于平衡状态，并且可以被300 nm以上的波长光解。两个系列都具有强荧光，测量了它们的发射和激发荧光光谱以及量子产率。核苷5'-三磷酸酯能够引发并维持猪脑微管蛋白的聚合。
