Leonard N J, Keyser G E
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4262-4. doi: 10.1073/pnas.76.9.4262.
The fluorescent nucleotide analogues (the 5'-mono-, di-, and triphosphates of lin-benzoguanosine, lin-benzoxanthosine, and lin-benzoinosine) have been prepared for use as dimensional probes of enzyme binding sites. They have quantum yields in aqueous solution of 0.39, 0.55, and 0.04 and fluorescent lifetimes of 6, 9, and approximately equal to 1.5 nsec, respectively. lin-Benzoinosine 5'-monophosphate is a substrate for xanthine oxidase (xanthine:oxygen oxidoreductase, EC 1.2.3.2), providing lin-benzoxanthosine 5'-monophosphate, and lin-benzoinosine 5'-diphosphate is a substrate for polynucleotide phosphorylase (polyribonucleotide:orthophosphate nucleotidyltransferase. EC 2.7.7.8), giving poly(lin-benzoinosinic acid). The benzologues of the purine diphosphates are substrates for pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40), which is used to prepare the triphosphates.
荧光核苷酸类似物（连苯并鸟苷、连苯并黄苷和连苯并肌苷的5'-单磷酸、二磷酸和三磷酸）已被制备用作酶结合位点的尺寸探针。它们在水溶液中的量子产率分别为0.39、0.55和0.04，荧光寿命分别为6、9和约1.5纳秒。连苯并肌苷5'-单磷酸是黄嘌呤氧化酶（黄嘌呤：氧氧化还原酶，EC 1.2.3.2）的底物，生成连苯并黄苷5'-单磷酸，连苯并肌苷5'-二磷酸是多核苷酸磷酸化酶（聚核糖核苷酸：正磷酸核苷酸基转移酶，EC 2.7.7.8）的底物，生成聚（连苯并肌苷酸）。嘌呤二磷酸的苯并类似物是丙酮酸激酶（ATP：丙酮酸2-O-磷酸转移酶，EC 2.7.1.40）的底物，该酶用于制备三磷酸。
