Konev S V, Katibnikov M A, Bandarin V A, Niamaa D, Barkovskiĭ E V
Biofizika. 1975 Jul-Aug;20(4):586-90.
Results are presented of measuring fibrinogen fluorescence parameters in temperature range of 20-80 degrees C at different pH of the solution. It was found that the temperature increase from 20 to 40 degrees C for solutions with pH of 4,5-9,3 were not accompanied by the conformational changes of fibrinogen macromolecules. In the temperature range of 40-50 degrees C for neutral solutions conformational reconstruction of fibrinogen of undenatured character took place. Temperature increase above 50-55 degrees C brings about significant structural changes of fibrinogen molecule which are of denaturation nature.
给出了在20-80摄氏度温度范围内、不同溶液pH值条件下测量纤维蛋白原荧光参数的结果。研究发现，对于pH值为4.5-9.3的溶液，温度从20摄氏度升高到40摄氏度时，纤维蛋白原大分子未发生构象变化。在40-50摄氏度温度范围内，中性溶液中的纤维蛋白原发生了未变性特征的构象重建。温度升高至50-55摄氏度以上会导致纤维蛋白原分子发生显著的结构变化，这些变化具有变性性质。
