Cooley W A, Clark J K, Stack M J
Veterinary Laboratories Agency, Central Veterinary Laboratory, Weybridge, Surrey, UK.
J Comp Pathol. 1998 Jan;118(1):41-9. doi: 10.1016/s0021-9975(98)80026-1.
Detergent- and proteinase K-treated extracts of grey matter were prepared from four regions of the brains of 106 sheep with scrapie, diagnosed clinically and by the demonstration of spongiform encephalopathy. The extracts were examined by electron microscopy for the presence of scrapie-associated fibrils and by Western immunoblotting for the disease-specific abnormal prion protein (PrPSc). As a diagnostic method, Western immunoblotting proved to be more sensitive than electron microscopy, the detection rates in the 106 sheep being 97 and 91% respectively (medulla), 99 and 76% (cerebellum), 95 and 88% (frontal cerebral cortex) and 93 and 61% (occipital cerebral cortex). Neither fibrils nor PrPSc could be detected in comparable brain extracts from 25 control sheep which had shown no clinical or histopathological evidence of scrapie.
从106只临床诊断为瘙痒病且经海绵状脑病证实的绵羊大脑的四个区域制备了经去污剂和蛋白酶K处理的灰质提取物。通过电子显微镜检查提取物中是否存在瘙痒病相关纤维，并通过蛋白质免疫印迹法检测疾病特异性异常朊病毒蛋白（PrPSc）。作为一种诊断方法，蛋白质免疫印迹法被证明比电子显微镜更敏感，在106只绵羊中，延髓的检测率分别为97%和91%，小脑为99%和76%，额叶大脑皮层为95%和88%，枕叶大脑皮层为93%和61%。在25只未表现出瘙痒病临床或组织病理学证据的对照绵羊的可比脑提取物中，未检测到纤维或PrPSc。
