Louis-Sylvestre C, Rand J H, Gordon R E, Salafia C M, Berkowitz R L
Department of Obstetrics, Gynecology, and Reproductive Science, Mount Sinai School of Medicine, New York, New York 10029, USA.
Am J Obstet Gynecol. 1998 Feb;178(2):287-93. doi: 10.1016/s0002-9378(98)80014-8.
Thus far there is no satisfactory treatment for preterm premature rupture of the amniotic membranes. To evaluate the use of platelets to seal the membranes, we investigated in vitro interactions between platelets and the membranes.
Platelet aggregation in the presence of amnion, chorion, and amniotic fluid was quantified. Platelet adhesion to the fetal membranes was investigated by electron microscopy. In addition, a model system was designed to evaluate the capacity of platelets to seal a standardized puncture in the membranes.
Platelets aggregated in response to amnion but not to chorion or amniotic fluid. Adhesion and activation occurred on connective tissues underlying amnion and chorion but not on the amniotic epithelium. Platelets sealed a puncture in fetal membranes, and the platelet plug was visualized by electron microscopy.
Exposed connective tissue of amniotic membranes was demonstrated to trigger platelet adhesion, aggregation, and activation, and platelets were shown to seal a standardized puncture in fetal membranes. Our results indicate that platelets might be useful for treatment of ruptured membranes after needle puncture.
迄今为止，对于早产胎膜早破尚无令人满意的治疗方法。为评估使用血小板封闭胎膜的效果，我们研究了血小板与胎膜之间的体外相互作用。
对在羊膜、绒毛膜和羊水存在的情况下血小板聚集情况进行定量分析。通过电子显微镜研究血小板与胎膜的黏附情况。此外，设计了一个模型系统来评估血小板封闭胎膜标准化穿刺口的能力。
血小板对羊膜有反应而聚集，但对绒毛膜或羊水无反应。黏附与激活发生在羊膜和绒毛膜下方的结缔组织上，而非羊膜上皮。血小板封闭了胎膜上的穿刺口，通过电子显微镜可观察到血小板凝块。
已证实胎膜暴露的结缔组织可触发血小板黏附、聚集和激活，且血小板可封闭胎膜上的标准化穿刺口。我们的结果表明，血小板可能有助于治疗针刺后的胎膜破裂。
