将单核细胞增生李斯特菌iap基因的单链构象多态性（SSCP）分析作为检测不同血清群的工具
Single-strand conformation polymorphism (SSCP) analysis of Listeria monocytogenes iap gene as tool to detect different serogroups.
作者信息
Manzano M, Cocolin L, Pipan C, Falasca E, Botta G A, Cantoni C, Comi G
机构信息
Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, Università di Udine, Italy.
出版信息
Mol Cell Probes. 1997 Dec;11(6):459-62. doi: 10.1006/mcpr.1997.0137.
PMID:9500812
Abstract
PCR-single-strand conformation polymorphism (PCR-SSCP) analysis is a convenient technique for the detection of mutations. As the mobility of single-stranded DNA is sequence-dependent it could therefore be used to determine serotype-related sequence variations in Listeria monocytogenes. Sero-specific patterns were observed in different L. monocytogenes serogroups.
摘要
聚合酶链反应-单链构象多态性（PCR-SSCP）分析是一种检测突变的便捷技术。由于单链DNA的迁移率取决于序列，因此它可用于确定单核细胞增生李斯特菌血清型相关的序列变异。在不同的单核细胞增生李斯特菌血清群中观察到了血清特异性模式。
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